Characterization of new GPCR signaling mechanisms involving WDR36 and Siva1

涉及 WDR36 和 Siva1 的新 GPCR 信号传导机制的表征

• 批准号: 238346-2013
• 负责人: Parent, JeanLuc
• 金额: $ 3.64万
• 依托单位: Université de Sherbrooke
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=546961
• 关键词: Characterization; new; GPCR; signaling; mechanisms

One way that cells communicate with each other is by sending chemical messages. These messages are picked up at the surface of the cells by specialized proteins that we refer to as "receptors". The receptors transduce the chemical messages to the interior of the cells and will induce activation or inactivation of a variety of intracellular proteins called "effectors" that cause changes in the function of the cells. The biggest family of receptors is formed by the G protein-coupled receptors (GPCRs) which transduce signals by the intermediary of a "G protein", hence their name. Through diffferent G proteins, a given GPCR can regulate the activity of various effectors. However, the mechanisms that determine the specificity and efficiency of coupling between a GPCR and its effectors are not well understood. We have discovered that proteins called WDR36 and Siva1 dictate the formation of specific signaling complexes between a GPCR called TPbeta, and particular effectors. The present research program will address in detail the molecular mechanisms involved in the regulation of intracellular signaling pathways by WDR36 and Siva1 that modulate cell physiology.

细胞之间交流的一种方式是发送化学信息。这些信息在细胞表面被我们称为“受体”的特殊蛋白质接收。受体将化学信息传递到细胞内部，并诱导多种细胞内蛋白质的激活或失活，这些蛋白质被称为“效应器”，导致细胞功能的变化。最大的受体家族是由G蛋白偶联受体（gpcr）组成的，它们通过“G蛋白”的中介传导信号，因此得名。给定的GPCR可以通过不同的G蛋白调控各种效应物的活性。然而，决定GPCR及其效应物之间偶联的特异性和效率的机制尚不清楚。我们已经发现，叫做WDR36和Siva1的蛋白质决定了一种叫做TPbeta的GPCR和特定效应物之间特定信号复合物的形成。目前的研究计划将详细讨论WDR36和Siva1调节细胞生理的细胞内信号通路的分子机制。