WDR36 as a scaffold protein for GPCR-Gq-PLCbeta signalling

WDR36 作为 GPCR-Gq-PLCbeta 信号传导的支架蛋白

• 批准号: 238346-2012
• 负责人: Parent, JeanLuc
• 金额: $ 2.04万
• 依托单位: Université de Sherbrooke
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=505809
• 关键词: WDR36; scaffold; protein; GPCR; Gq

One way that cells communicate with each other is by sending chemical messages. These messages are picked up at the surface fo the cells by specialized proteins that we refer to as "receptors". The receptors transduce the chemical messages to the interior of the cells and will induce activation or inactivation of a variety of intracellular proteins called "effectors" that cause changes in the function of the cells. The biggest family of receptors is formed by the G protein-coupled receptors (GPCRs) which transduce signals by the intermediary of a "G protein", hence their name. Through diffferent G proteins, a given GPCR can regulate the activity of various effectors. However, the mechanisms that determine the specificity and efficiency of coupling between a GPCR and a particular G protein and effector are not well understood. We have discovered that a protein called WDR36 dictates the formation of a specific complex between a GPCR called TPbeta, a G protein (Gq) and an effector (PLCbeta). The present research program will address in detail the mechanisms involved in the function of WDR36 and how it affects other signalling pathways in the cells.

细胞之间相互交流的一种方式是发送化学信息。这些信息是由我们称为“受体”的特殊蛋白质在细胞表面拾取的。受体将化学信息传递到细胞内部，并将引起细胞内各种被称为“效应器”的蛋白质的激活或失活，从而导致细胞功能的变化。最大的受体家族是由G蛋白偶联受体(GPCRs)组成的，它通过“G蛋白”的中介传递信号，因此得名。通过不同的G蛋白，一个特定的GPCR可以调节各种效应器的活性。然而，决定GPCR与特定G蛋白和效应器之间偶联的特异性和效率的机制还不是很清楚。我们已经发现，一种名为WDR36的蛋白质决定着在名为TPbeta的GPCR、G蛋白(GQ)和效应器(PLCbeta)之间形成一种特定的复合体。目前的研究计划将详细阐述WDR36功能涉及的机制以及它如何影响细胞中的其他信号通路。