Aptamer-facilitated technologies for cell activity modulation and imaging

用于细胞活性调节和成像的适体促进技术

• 批准号: 385739-2010
• 负责人: Berezovski, Maxim
• 金额: $ 2.55万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=550255
• 关键词: Aptamer; facilitated; technologies; cell; activity

One of the most challenging tasks of bioanalytical chemistry and biotechnology is the replacement of animal- or hybridoma-made antibodies with artificial competitors that can be easily selected and chemically synthesized. Such competitors are aptamers - single-stranded DNA or RNA oligonucleotides that can bind to a variety of different targets ranging from small molecules to proteins with high affinity and specificity. Aptamers are ligands with big potential to change the field of affinity probes and to replace antibodies as diagnostic and therapeutic reagents. In contrast to antibodies, they do not trigger an immune response. They are thermostable and can be chemically synthesized for lower price, have better batch-to-batch reproducibility, and have the simplicity of chemical modifications and integration in biosensors.

生物分析化学和生物技术最具挑战性的任务之一是用易于选择和化学合成的人工竞争剂替代动物或杂交瘤制造的抗体。这些竞争者是适体——单链 DNA 或 RNA 寡核苷酸，可以以高亲和力和特异性结合各种不同的靶标，从小分子到蛋白质。适体是具有巨大潜力的配体，可以改变亲和探针领域并取代抗体作为诊断和治疗试剂。与抗体相反，它们不会引发免疫反应。它们具有热稳定性，可以以较低的价格进行化学合成，具有更好的批次重现性，并且化学修饰和生物传感器集成简单。