Robotic selection of DNA aptamers to powdery mildew

白粉病，也被称为白粉病，是由真菌钩端螺旋体引起的。这种真菌有一条狭窄的 寄主范围只攻击葡萄植物和少数相关物种。它是最常见和最普遍的疾病。 加拿大不列颠哥伦比亚省奥卡纳根/西米尔卡明地区的葡萄藤。毫无疑问，这是 葡萄生产者面临的最持久、最持久的疾病问题。 在这项提案中，别列佐夫斯基的实验室和Flex Alert公司将开发白粉病的DNA适配子 使用机器人平台。适配子是一种特定的核酸序列，可以与多种靶标结合 具有很高的亲和力和特异性。它们可以被化学修饰并结合到各种简单的 病原体检测方法，包括流式细胞术、细胞成像和适体传感器。已开发的 适体可以应用于智能食品包装的低成本生物传感器，用于检测易腐烂的病原体。 水果和葡萄酒生产。传感器技术是基于适体沉积在塑料薄膜上的 连接到无线网络，允许通过使用移动设备进行监控、跟踪 网络和智能手机。

Powdery mildew, also known as oidium, is caused by the fungus Uncinula necator. This fungus has a narrow host range attacking only grape plants and a few related species. It is the most common and widespread disease of grapevines in the Okanagan/Similkameen area of British Columbia in Canada. It is, without a doubt, the most enduring and persistent disease problem faced by grape producers. In this proposal, Berezovski's lab and Flex Alert Company will develop DNA aptamers for powdery mildew using a robot platform. Aptamers are specific nucleic acid sequences that can bind to a wide range of targets with high affinity and specificity. They can be chemically modified and incorporated into a variety of simple assays for pathogen detection, including flow cytometry, cell imaging, and aptasensors. The developed aptamers can be applied in low cost biosensors for smart food packaging, for detecting pathogens in perishable fruits and wine production. The sensor technology is based on the deposition of aptamers on plastic films with a connection to a wireless network, which allows the monitoring, tracking through the use of mobile networking and smart phones.