Interaction dynamics and bioeffects of acoustically activated microbubbles with biological membranes

声激活微泡与生物膜的相互作用动力学和生物效应

• 批准号: 386274-2010
• 负责人: Karshafian, Raffi
• 金额: $ 1.82万
• 依托单位: Ryerson University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=550353
• 关键词: Interaction; dynamics; bioeffects; acoustically; activated

Ideally, therapeutic drugs would be delivered entirely to diseased tissues, sparring healthy tissue. However, existing therapeutic drugs generally interact with both diseased and healthy tissues. Treatment effectiveness of cytotoxic drugs such as chemotherapy depends on the extent to which their constituents penetrate tissues and cells to reach their intended target and is limited by toxic side effects exerted on tissues and cells not associated with the disease. Of particular interest to our group is the ability of sound energy in combination with tiny bubbles, which are smaller than red blood cells, to non-invasively improve delivery of drugs to diseased cells and tissues deep with the body. Our group will examine the interaction of sound energy with bubbles and their effect on live cells with the aim of answering fundamental questions on the biophysical mechanisms involved, as this remains a subject of considerable investigation. Bubbles, exposed to sound energy at megahertz frequency (millions of oscillation per second) that undergo oscillation and/or violent collapse, will be characterized using acoustic methods and a high-speed camera (500,000 frames per second). Cells will be analyzed using a variety of biological methods. Improving our understanding of the processes underpinning the ability of ultrasound and bubbles to selectively and locally deliver therapeutic agents will allow optimization of drug-treatments and also lead to a better understanding of the strengths and limitations of this technology - ultrasound targeted delivery of drugs - with regards to its clinical use.

理想情况下，治疗药物将完全输送到患病组织，而健康组织则不会受到影响。 然而，现有的治疗药物通常与患病组织和健康组织两者相互作用。 细胞毒性药物如化疗的治疗效果取决于其成分渗透组织和细胞以到达其预期靶点的程度，并且受到对与疾病无关的组织和细胞施加的毒副作用的限制。 我们小组特别感兴趣的是声能与比红细胞小的微小气泡相结合的能力，以非侵入性方式改善药物向身体深处的患病细胞和组织的输送。 我们的小组将研究声能与气泡的相互作用及其对活细胞的影响，目的是回答有关生物物理机制的基本问题，因为这仍然是一个相当大的研究课题。 暴露于兆赫频率（每秒数百万次振荡）的声能并经历振荡和/或剧烈崩溃的气泡将使用声学方法和高速相机（每秒500，000帧）进行表征。 将使用各种生物学方法分析细胞。 提高我们对超声波和气泡选择性和局部递送治疗剂能力的基础过程的理解，将允许优化药物治疗，并更好地理解这种技术的优势和局限性-超声波靶向药物递送-关于其临床用途。