Regulation of cyclic nucleotide hydrolysis in bovine sperm

牛精子环核苷酸水解的调控

• 批准号: 435507-2012
• 负责人: Richard, François
• 金额: $ 5.92万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=552782
• 关键词: Regulation; cyclic; nucleotide; hydrolysis; bovine

Many functions of sperm physiology are regulated by cyclic nucleotides. Specifically, cyclic adenosine monophosphate is involved in crucial events in sperm maturation processes such as motility, capacitation and acrosome reaction. Cyclic nucleotides are synthesized by cyclases to reach a threshold level activating kinases, phosphodiesterases (PDE) and ion gated-channels. Intracellular content of cyclic nucleotides depends not only on synthesis but on degradation by PDE. We have developed a test measuring PDE activity in bovine spermatozoa which supports the functional presence of one specific family, PDE10A. This grant application is aiming to further demonstrate that these technologies will offer a commercial advantage for Canadian insemination industries by optimizing the use of bovine spermatozoa, a market of several million dollars.

精子的许多生理功能都受到环核苷酸的调节。具体而言，环磷酸腺苷参与精子成熟过程中的关键事件，如运动，获能和顶体反应。环核苷酸由环化酶合成以达到激活激酶、磷酸二酯酶（PDE）和离子门控通道的阈值水平。环核苷酸的细胞内含量不仅取决于合成，而且取决于PDE的降解。我们已经开发了一种测量牛精子中PDE活性的测试，该测试支持一个特定家族PDE 10A的功能存在。这项赠款申请旨在进一步证明这些技术将通过优化牛精子的使用为加拿大授精行业提供商业优势，这是一个数百万美元的市场。