In vitro translation of in vivo stimulation

体内刺激的体外转化

• 批准号: 417841-2011
• 负责人: Richard, François
• 金额: $ 5.69万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=530957
• 关键词: vitro; translation; vivo; stimulation

Known as an international leader, Canada has a long history of collaborative efforts between researchers from universities, industries and agencies. In spite of embryonic production is feasible for several decades; the yield is still not optimal especially when oocytes are from small follicular diameters. Even if several months are required for the growth of the ovarian follicles, the crucial events for developmental competence acquisition are occurring around the final maturation of the follicles, a step happening before ovulation. One of the proposed strategies to improve this competence is to influence the oocyte with the late follicular signalling while the oocyte is maintained in meiotic arrest as it occurs naturally in the follicular environment. We have recently developed the so-called "Stop-Egg-Go" as a physiological treatment to efficiently maintain bovine oocytes in meiotic arrest. This approach is of high commercial relevance since it provides a critical window to improve developmental competence of oocytes. In addition, it will allow the transport of oocytes to the laboratory, a key alternative for the expansion of reproductive technologies in dairy cow market.

作为国际领导者，加拿大在大学，工业和机构的研究人员之间的合作努力方面有着悠久的历史。尽管胚胎生产在几十年内是可行的，但产量仍然不是最佳的，特别是当卵母细胞来自小卵泡直径时。即使卵泡的生长需要几个月的时间，发育能力获得的关键事件也发生在卵泡的最终成熟周围，这是排卵前发生的一个步骤。提出的提高这种能力的策略之一是用晚期卵泡信号传导影响卵母细胞，同时卵母细胞维持在减数分裂停滞中，因为它在卵泡环境中自然发生。我们最近开发了所谓的“Stop-Egg-Go”作为一种生理处理，以有效地维持牛卵母细胞减数分裂停滞。这种方法具有高度的商业相关性，因为它提供了改善卵母细胞发育能力的关键窗口。此外，它将允许将卵母细胞运输到实验室，这是扩大奶牛市场生殖技术的关键替代方案。