Molecular Interactions and Structural Biology

分子相互作用和结构生物学

• 批准号: 1000227085-2011
• 负责人: Boulanger, Martin
• 金额: $ 7.29万
• 依托单位: University of Victoria
• 依托单位国家: 加拿大
• 项目类别: Canada Research Chairs
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=557469
• 关键词: Molecular; Interactions; Structural; Biology

In a letter to T.H. Huxley, Charles Darwin famously commented that: "The time will come, I believe, though I will not live to see it, when we shall have very nearly true genealogical trees of each great kingdom of nature...". It now seems possible that we will realize Darwin's dream within our lifetimes. Indeed, one of the most exciting long-standing problems in evolutionary biology has been to determine what exactly are the 'great kingdoms of nature'. In the last few decades, by applying molecular biology and genomic techniques to understanding the tree of Life we have found that kingdoms such as the animals, fungi and plants represent only a small corner of the diversity of Life. The deepest evolutionary divergences amongst nucleus-containing organisms (eukaryotes) involve a wide array of single-celled organismal lineages called 'protists'. However, the relationships amongst these ancient evolutionary lineages have, until recently, remained elusive. In this proposal we will use high-throughput DNA sequencing technology to characterize the expressed portion of the genomes of ten newly-described single celled organisms. By comparing these data to similar data from dozens of better characterized organisms representing the full breadth of biological diversity using sophisticated computational methods, we can delineate the super-kingdoms of Life. In so doing, we will clarify how and when some of the major 'transitions' in evolutionary history of Life took place on the ancient Earth.

在给T. H的一封信中。赫胥黎，查尔斯达尔文的著名评论：“我相信，虽然我不会活着看到它，但时间会到来，当我们拥有每一个伟大的自然王国的非常接近真实的家谱树时。".现在看来，我们有可能在有生之年实现达尔文的梦想。事实上，进化生物学中最令人兴奋的长期存在的问题之一就是确定什么是“大自然王国”。在过去的几十年里，通过应用分子生物学和基因组技术来理解生命之树，我们发现动物、真菌和植物等王国只代表了生命多样性的一个小角落。含核生物（真核生物）之间最深的进化分歧涉及广泛的单细胞生物谱系，称为“原生生物”。然而，直到最近，这些古老的进化谱系之间的关系仍然难以捉摸。在这个提议中，我们将使用高通量DNA测序技术来表征十个新描述的单细胞生物的基因组的表达部分。通过使用复杂的计算方法将这些数据与代表生物多样性的数十种更好表征的生物体的类似数据进行比较，我们可以描绘出生命的超级王国。 在这样做的时候，我们将澄清生命进化史上的一些主要“转变”是如何以及何时在古代地球上发生的。