Monitoring active biomass in biofilms by ATP measurement

通过 ATP 测量监测生物膜中的活性生物量

• 批准号: 469580-2014
• 负责人: Lee, Hung
• 金额: $ 1.82万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=563590
• 关键词: Monitoring; active; biomass; biofilms; ATP

Biological treatment of wastewater relies on the action of diverse competent microorganisms to degrade the organic matter. Many of these microorganisms are found as surface associated biofilms or suspended microbial flocs. Bacterial biofilms are communities of cells attached to surfaces where nutrients and other cells aggregate, and thus are locations where active metabolism occurs. Wastewater treatment plants (WWTP) are potential hotspots for the accumulation of many antimicrobial agents, such as antibiotics. However, the peculiar nature of biofilms shelter microorganisms from antibiotics or other inhibitory substances. Rapid Biodetection Inc. is a privately owned Canadian company that specializes in real-time monitoring systems for drinking water distribution networks and wastewater treatment. They provide rapid detection systems that are applied in different fields from monitoring water quality in distribution networks, wastewater treatment plants, and water in oil and gas industries. To evaluate the active microbial component, Rapid Biodetection utilizes an adenosine triphosphate (ATP) based assay to measure the living biomass activity for the water system being tested. While there is considerable information on the suitability of the ATP assay to measure living microbial cells in liquid microbial cell suspension, less research has been undertaken to assess the living microbial biomass in biofilms or clumped in flocs. In this proposal, we will use the ATP test provided by Rapid Biodetection to assess the effect of antimicrobial agents on the active biomass found in biofilms generated in the laboratory. The results will be compared to other means of assessing active microbial biomass and

废水的生物处理依赖于各种活性微生物的作用来降解有机物。这些微生物中有许多是表面相关的生物膜或悬浮的微生物絮凝体。细菌生物膜是附着在营养物质和其他细胞聚集的表面上的细胞群落，因此是活跃代谢发生的地方。污水处理厂是抗生素等抗菌药物积累的潜在热点。然而，生物膜的特殊性质使微生物免受抗生素或其他抑制物质的侵害。Rapid Biodetection Inc.是一家加拿大私营公司，专门从事饮用水分配网络和废水处理的实时监测系统。他们提供快速检测系统，应用于不同领域，从监测配电网、污水处理厂到石油和天然气行业的水质。为了评估活性微生物成分，快速生物检测利用基于三磷酸腺苷（ATP）的测定来测量被测水系统的活生物量活性。虽然关于ATP测定在液体微生物细胞悬浮液中测量活微生物细胞的适用性有相当多的信息，但对生物膜或絮凝体中活微生物生物量的评估研究较少。在本提案中，我们将使用Rapid Biodetection提供的ATP测试来评估抗菌药物对实验室生成的生物膜中活性生物质的影响。结果将与其他评估活性微生物生物量的方法进行比较