Monitoring active biomass in biofilms by ATP measurement

废水的生物处理依赖于多种活性微生物的作用来降解有机物。这些微生物中的许多被发现作为表面相关的生物膜或悬浮的微生物絮凝物。细菌生物膜是附着在营养物和其他细胞聚集的表面上的细胞群落，因此是发生活性代谢的位置。污水处理厂（WWTP）是许多抗菌剂（如抗生素）积累的潜在热点。然而，生物膜的特殊性质使微生物免受抗生素或其他抑制物质的侵害。快速生物检测公司是一家私营的加拿大公司，专门从事饮用水分配网络和废水处理的实时监控系统。它们提供快速检测系统，应用于不同领域，从监测配电网，污水处理厂的水质到石油和天然气行业的水。为了评估活性微生物成分，快速生物检测利用基于三磷酸腺苷（ATP）的测定来测量被测水系统的活生物量活性。虽然有相当多的信息ATP测定法测量液体微生物细胞悬浮液中的活微生物细胞的适用性，很少有研究已经进行，以评估生物膜中的活微生物生物量或聚集在絮凝物。在本提案中，我们将使用Rapid Biodetection提供的ATP测试来评估抗菌剂对实验室产生的生物膜中发现的活性生物量的影响。将结果与评估活性微生物生物量的其他方法进行比较，

Biological treatment of wastewater relies on the action of diverse competent microorganisms to degrade the organic matter. Many of these microorganisms are found as surface associated biofilms or suspended microbial flocs. Bacterial biofilms are communities of cells attached to surfaces where nutrients and other cells aggregate, and thus are locations where active metabolism occurs. Wastewater treatment plants (WWTP) are potential hotspots for the accumulation of many antimicrobial agents, such as antibiotics. However, the peculiar nature of biofilms shelter microorganisms from antibiotics or other inhibitory substances. Rapid Biodetection Inc. is a privately owned Canadian company that specializes in real-time monitoring systems for drinking water distribution networks and wastewater treatment. They provide rapid detection systems that are applied in different fields from monitoring water quality in distribution networks, wastewater treatment plants, and water in oil and gas industries. To evaluate the active microbial component, Rapid Biodetection utilizes an adenosine triphosphate (ATP) based assay to measure the living biomass activity for the water system being tested. While there is considerable information on the suitability of the ATP assay to measure living microbial cells in liquid microbial cell suspension, less research has been undertaken to assess the living microbial biomass in biofilms or clumped in flocs. In this proposal, we will use the ATP test provided by Rapid Biodetection to assess the effect of antimicrobial agents on the active biomass found in biofilms generated in the laboratory. The results will be compared to other means of assessing active microbial biomass and