A laser-scanning system for radioisotope-labeled, multi-fluorescent, chemifluorescent and colorimetric imaging

用于放射性同位素标记、多荧光、化学荧光和比色成像的激光扫描系统

• 批准号: 472898-2015
• 负责人: Zimmerly, Steven
• 金额: $ 9.33万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=564876
• 关键词: laser; scanning; system; radioisotope; labeled

Funds are requested to purchase a general-purpose, state-of-the-art laser scanning imaging system, to be used by multiple research programs in the Department of Biological Sciences. The instrument is a replacement and upgrade of an existing scanning system that has become inoperable and nonfunctional. The proposed instrument has broad applicability for many research purposes, being capable of detecting radiolabeled molecules through phosphorimaging (e.g., P-32, S-35, C-14), and detecting fluorescence signals with blue, green or red lasers, thereby producing images or quantitating data with high accuracy over five orders of magnitude of signal. Experimental techniques enabled by the instrument include the following: biochemical assays and hybridization blots based on radioactive detection; Southern, northern and western blots based on fluorescence detection, scanning of fluorescence data from glass slides or microtiter plates with a resoution of 10 micrometers; 2D DIGE (differential gel electrophoresis) for proteomic applications; and quantitation of visibly stained gels (Coomassie blue, silver). The eight applying labs have quite diverse research programs in the fields of RNA biology (group II introns and reverse transcriptases), structural biology (viral polymerases), plant metabolism (natural bioproduct chemistry), plant physiology (cell signaling, growth), yeast cell biology (cell wall structure and synthesis), developmental biology (limb development of mice), stem cell biology (germ line differentiation in worm) and cancer biology (breast stem cells). Additional labs in the building and department are expected to utilize the instrument because of its great versatility and applicability for many research purposes.

需要资金购买通用的、最先进的激光扫描成像系统，供生物科学系的多个研究项目使用。该仪器是现有扫描系统的替代和升级，该系统已无法操作且无法运行。该仪器对许多研究目的具有广泛的适用性，能够通过磷光成像（例如，P-32、S-35、C-14）检测放射性标记分子，并用蓝色、绿色或红色激光检测荧光信号，从而产生超过五个数量级信号的高精度图像或定量数据。该仪器支持的实验技术包括：基于放射性检测的生化测定和杂交印迹；基于荧光检测的 Southern、Northern 和 Western 印迹，扫描载玻片或微量滴定板的荧光数据，分辨率为 10 微米；用于蛋白质组学应用的 2D DIGE（差示凝胶电泳）；以及对明显染色的凝胶（考马斯蓝、银）进行定量。八个申请实验室在RNA生物学（II类内含子和逆转录酶）、结构生物学（病毒聚合酶）、植物代谢（天然生物产品化学）、植物生理学（细胞信号传导、生长）、酵母细胞生物学（细胞壁结构和合成）、发育生物学（小鼠肢体发育）、干细胞生物学（线虫种系分化）和癌症领域拥有相当多样化的研究项目 生物学（乳腺干细胞）。由于该仪器具有强大的多功能性和适用性，可用于许多研究目的，因此大楼和部门的其他实验室预计将使用该仪器。