Natural diversity of group II introns

移动的DNA是自私的遗传元件，它们的增殖不会立即给宿主带来好处;然而，随着时间的推移，它们已经成为许多有用的进化发明的来源，如端粒酶。移动的DNA是诱变剂，但也是适应的使能者，其动力学尚未得到很好的理解。II组内含子是细菌中一类移动的DNA，具有催化RNA和逆转录元件的性质。内含子的序列编码两种组分：1）进行自剪接反应的催化RNA组分;和2）促进剪接反应并允许内含子整合到新的基因组位置的内含子编码的逆转录酶。II组内含子是非常通用的，已经进化成许多变体形式，其中最重要的可以说是真核剪接体内含子和非LTR反转录转座子，它们分别占人类基因组的~25%和~20%。Zimmerly实验室过去的研究有助于确定自然界中存在的第二组内含子的宽度。在这里，我们将进一步研究第二组内含子如何分化和进化，以获得新的特征和生物学影响，有三个目标。1)我们将使用生物信息学工具在GenBank中筛选II组内含子。除了生成更新的普查，预计这将确定新的内含子变体。系统发育分析积累的序列数据将允许重建的内含子的主要谱系的进化。2)我们将研究二进制剪接的现象，这是由第二组内含子的子集进行。虽然大多数II组内含子通过单个外显子结合基序识别它们的5'外显子，但一个子集可以交替使用两个外显子结合基序中的任一个。我们将剖析二元剪接的机制，并确定其对内含子迁移率的影响，我们假设这是不寻常的剪接特性的生物学功能。3)我们将研究C.te.I1内含子的选择性剪接的前所未有的反应，

Mobile DNAs are selfish genetic elements that proliferate without immediate benefits to their hosts; however, over time they have been the source of many useful evolutionary inventions such as telomerase. The dynamics by which mobile DNAs are agents of mutagenesis, yet also enablers of adaptation, are not well understood. Group II introns are a type of mobile DNA in bacteria that possesses properties of both catalytic RNAs and retroelements. The sequence of the introns encodes two components: 1) a catalytic RNA component that carries out a self-splicing reaction; and 2) an intron-encoded reverse transcriptase that facilitates the splicing reaction and allows the introns to integrate into new genomic locations. Group II introns are exceptionally versatile, having evolved into many variant forms, with the most important arguably being eukaryotic spliceosomal introns and non-LTR retrotransposons, which make up ~25% and ~20% of the human genome, respectively. Past research in the Zimmerly lab has helped to establish the breadth of group II introns that exist in nature. Here we will further investigate how group II introns have differentiated and evolved to acquire new characteristics and biological impacts, with three aims. 1) We will use bioinformatic tools to curate group II introns in GenBank. In addition to generating an updated census, this is expected to identify new intron variants. Phylogenetic analysis of the accumulated sequence data will allow reconstruction of the evolution of a major lineage of the introns. 2) We will investigate the phenomenon of binary splicing, which is carried out by a subset of group II introns. While most group II introns recognize their 5’ exon by a single exon-binding motif, a subset can alternately use either of two exon-binding motifs. We will dissect the mechanism for binary splicing and determine its effect on intron mobility, which we hypothesize to be the biological function of the unusual splicing property. 3) We will examine the unprecedented reaction of alternative splicing of the C.te.I1 intron in