Natural diversity of group II introns

II 组内含子的自然多样性

• 批准号: RGPIN-2017-05871
• 负责人: Zimmerly, Steven
• 金额: $ 3.64万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=669944
• 关键词: Natural; diversity; group; II; introns

Mobile DNAs are selfish genetic elements that proliferate without immediate benefits to their hosts; however, over time they have been the source of many useful evolutionary inventions such as telomerase. The dynamics by which mobile DNAs are agents of mutagenesis, yet also enablers of adaptation, are not well understood. Group II introns are a type of mobile DNA in bacteria that possesses properties of both catalytic RNAs and retroelements. The sequence of the introns encodes two components: 1) a catalytic RNA component that carries out a self-splicing reaction; and 2) an intron-encoded reverse transcriptase that facilitates the splicing reaction and allows the introns to integrate into new genomic locations. Group II introns are exceptionally versatile, having evolved into many variant forms, with the most important arguably being eukaryotic spliceosomal introns and non-LTR retrotransposons, which make up ~25% and ~20% of the human genome, respectively. Past research in the Zimmerly lab has helped to establish the breadth of group II introns that exist in nature. Here we will further investigate how group II introns have differentiated and evolved to acquire new characteristics and biological impacts, with three aims. 1) We will use bioinformatic tools to curate group II introns in GenBank. In addition to generating an updated census, this is expected to identify new intron variants. Phylogenetic analysis of the accumulated sequence data will allow reconstruction of the evolution of a major lineage of the introns. 2) We will investigate the phenomenon of binary splicing, which is carried out by a subset of group II introns. While most group II introns recognize their 5' exon by a single exon-binding motif, a subset can alternately use either of two exon-binding motifs. We will dissect the mechanism for binary splicing and determine its effect on intron mobility, which we hypothesize to be the biological function of the unusual splicing property. 3) We will examine the unprecedented reaction of alternative splicing of the C.te.I1 intron in Clostridium tetani. Here an intron has taken on a presumably beneficial role, allowing a single 5' exon to be ligated to one of four downstream exons, producing five protein variants from a single genetic locus. To address the biological consequences of this reaction, we will investigate the intron in three patient isolates of C. tetani and compare data to a reference strain. The genomes of the three strains will be sequenced, and RNA-Seq will be used to detect splicing and transcription properties. Together these three approaches will help illuminate the functional variants and adaptations of group II introns, and contribute to our understanding of how mobile DNAs and ribozymes adapt and diversify over evolutionary time, sometimes becoming domesticated to take on useful roles within their bacterial hosts.

移动的DNA是自私的遗传元件，它们的增殖不会立即给宿主带来好处;然而，随着时间的推移，它们已经成为许多有用的进化发明的来源，如端粒酶。移动的DNA是诱变剂，但也是适应的使能者，其动力学尚未得到很好的理解。II组内含子是细菌中一类移动的DNA，具有催化RNA和逆转录元件的性质。内含子的序列编码两种组分：1）进行自剪接反应的催化RNA组分;和2）促进剪接反应并允许内含子整合到新的基因组位置的内含子编码的逆转录酶。II组内含子是非常通用的，已经进化成许多变体形式，其中最重要的可以说是真核剪接体内含子和非LTR反转录转座子，它们分别占人类基因组的~25%和~20%。Zimmerly实验室过去的研究有助于确定自然界中存在的第二组内含子的宽度。在这里，我们将进一步研究第二组内含子如何分化和进化，以获得新的特征和生物学影响，有三个目标。1)我们将使用生物信息学工具在GenBank中筛选II组内含子。除了生成更新的普查，预计这将确定新的内含子变体。系统发育分析积累的序列数据将允许重建的内含子的主要谱系的进化。2)我们将研究二元剪接现象，该现象由II组内含子的子集进行。虽然大多数II组内含子通过单个外显子结合基序识别它们的5'外显子，但一个子集可以交替使用两个外显子结合基序中的任一个。我们将剖析二元剪接的机制，并确定其对内含子迁移率的影响，我们假设这是不寻常的剪接特性的生物学功能。3)我们将研究破伤风梭菌中C.te.I1内含子的选择性剪接的前所未有的反应。在这里，内含子已经承担了一个可能有益的作用，允许单个5'外显子连接到四个下游外显子中的一个，从一个遗传位点产生五种蛋白质变体。为了解决这种反应的生物学后果，我们将研究三个病人分离的C。并将数据与参考菌株进行比较。将对三种菌株的基因组进行测序，并将使用RNA-Seq检测剪接和转录特性。这三种方法将有助于阐明II组内含子的功能变体和适应性，并有助于我们理解移动的DNA和核酶如何在进化过程中适应和多样化，有时会被驯化，在细菌宿主中发挥有用的作用。