Plant transcriptomic and metabolomic core platform

植物对环境作出反应，调节基因的转录，以最大限度地提高生存和生长。转录组变化的结果导致各种蛋白质被表达，以制造适合植物环境中普遍存在的化合物。这些化合物被称为代谢物，代表了植物中存在的数百万分子的强大储存库。建议中所要求的设备旨在实现以下两个目标；1)监测植物基因表达2)量化植物产生的各种代谢物的数量和多样性。需要这些设备的两个实验室由年轻教授（分别于2012年和2013年聘用）领导，他们的研究项目由NSERC （Discovery and Engage）、FRQNT （Nouveau chercheur）和一位机构研究主席（UQTR）资助。虽然两位PI都成功地获得了运营资助，但他们（和他们的部门）缺乏足够的设备来监测基因表达和代谢组变异，尽管这些简单的设备是他们的发现研究计划的基础。这两个研究项目都使用基于研究的系统生物学，如转录组和代谢组数据的整合来阐明不同的植物过程。具体来说，Germain博士的研究项目侧重于植物对生物营养病原体的免疫反应，这一过程涉及由植物激素水杨酸调节的几个基因，其水平只能通过高效液相色谱法监测。desgagn<s:1> - penix博士的研究旨在阐明非模式植物中的生物化学途径，特别是异喹啉生物碱的生物合成（例如治疗阿尔茨海默病的药物加兰他明、抗癌剂水仙素和抗菌化合物克里宁）。成功阐明这些途径需要比较产生（或不产生）给定代谢物生物碱（本身由HPLC监测）的植物之间的基因表达。因此，正如他们在发现基金中概述的那样，基因表达和代谢物分析对他们的研究项目至关重要。

Plants react to their environment and modulate the transcription of their genes to maximize survival and growth. The outcomes of transcriptomic changes results in various proteins being expressed to manufacture the compounds befitting the situation prevailing in the plant environment. Such compounds are termed metabolites and represent a formidable reservoir of millions of molecules present in plants. The equipment requested in the proposal aims the two following goals; 1) monitor plant gene expression 2) quantify the amount and diversity of various metabolites produced by plants. The two laboratories requesting the equipment are led by young professors (hired in 2012 and 2013 respectively) whose research programs are funded by NSERC (Discovery and Engage), FRQNT (Nouveau chercheur) and an institutional research chair (UQTR). While both PI have been successful at obtaining operating grant, they (and their department) lack the adequate equipment to monitor gene expression and metabolome variation despite the fact that these simple equipment are at the basis of their Discovery research program. Both research programs use research-based systems biology such as the integration of transcriptome and metabolome data to elucidate different plant processes. Specifically, Dr. Germain's research program focuses on plant immune response to biotrophic pathogen a process involving the implication of several genes regulated by the phytohormone salicylic acid whose level can only be monitored by HPLC. Dr. Desgagné-Penix research aims the elucidation of biochemical pathways, specifically the biosynthesis of isoquinoline alkaloids (e.g. galanthamine, an Alzheimer's disease drug treatment; narciclasine, an anti-cancer agent; and crinine, an antimicrobial compound) in non-model plants. Successful elucidation of such pathways requires the comparison of gene expression between plant producing (or not) the given metabolite alkaloid (itself monitored by HPLC). Hence, as outlined in their Discovery grant, gene expression and metabolite profiling is crucial to their research program.