Growth in the host: catabolic capacities of opportunistic bacteria and possible links to virulence

宿主中的生长：机会性细菌的分解代谢能力及其与毒力的可能联系

• 批准号: 327054-2011
• 负责人: Cardona, Silvia
• 金额: $ 2.62万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=571905
• 关键词: Growth; host; catabolic; capacities; opportunistic

Bacteria are ubiquitous in nature, present in great diversity and quantity in all soils and water. Many environmental bacteria are harmless to animals and man, but others can jump from the environment to the human host and become extremely pathogenic. Infections with the soil bacteria Burkholderia cepacia complex have become a serious risk for people with the genetic disorder cystic fibrosis. We propose that that these opportunistic bacteria utilize nutrients to produce signal molecules that have a pathogenic effect to the host. In this research program, we will investigate which compounds present in the soil and in the human host serve as nutrients and at the same time elicit a pathogenic response in Burkholderia cepacia complex. We will create a large collection of bacterial mutants, defective in the use of certain nutrients and investigate if these mutants are less pathogenic. Thanks to B. cepacia complex's ability to colonize diverse hosts, we will be able to use host models like the plant Arabidopsis and the worm C. elegans, which allow screening hundreds of mutants in a short period of time. Our research will contribute to our general understanding of the host as a growth environment where the host nutrients could be exploited to build molecules that trigger pathogenic responses in opportunistic bacteria.

细菌在自然界中无处不在，在所有土壤和水中都存在着巨大的多样性和数量。许多环境细菌对动物和人类无害，但其他细菌可以从环境跳到人类宿主并变得非常致病。 土壤细菌洋葱伯克霍尔德氏菌复合体的感染已成为遗传性疾病囊性纤维化患者的严重风险。我们认为，这些机会细菌利用营养物质产生信号分子，对宿主有致病作用。 在这项研究计划中，我们将研究哪些化合物存在于土壤和人类宿主中作为营养物质，同时引起洋葱伯克霍尔德菌复合体的致病反应。我们将创造大量的细菌突变体，在使用某些营养素方面有缺陷，并调查这些突变体是否致病性较低。多亏了B。洋葱复合体的能力，殖民地不同的主机，我们将能够使用主机模型，如植物拟南芥和蠕虫C。elegans，它允许在短时间内筛选数百个突变体。 我们的研究将有助于我们对宿主作为生长环境的一般理解，在这种生长环境中，宿主营养物质可以被利用来构建引发机会细菌致病反应的分子。