Testis-specific isozyme of angiotensin converting enzyme (tACE) as a fertility marker in bulls

睾丸特异性血管紧张素转化酶（tACE）同工酶作为公牛生育力标志物

• 批准号: 452216-2013
• 负责人: Thundathil, Jacob
• 金额: $ 3.39万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=575469
• 关键词: Testis; specific; isozyme; angiotensin; converting

Even a modest increase in the reproductive rate in cattle substantially enhances productivity. Therefore, research to improve reproductive efficiency is important for the dairy industry. Fertility varies 20-25% among frozen semen samples classified as satisfactory (based on traditional semen evaluation), implying there are submicroscopic differences in the structure and function of sperm from elite bulls maintained in commercial AI centers. Understanding the molecular basis of these variations in the fertilizing ability of sperm will have numerous applications in the dairy industry, as it will enable us to: 1) incorporate the genetic basis of variations in sperm function into bull selection models to select bulls with high fertility at their earliest possible age; 2) determine novel strategies for bull calf management (for example, nutrition) for modulating the expression of genes regulating fertility; and 3) identify molecular markers for fertility which will enable AI industries to develop laboratory tests for precisely documenting fertility of frozen semen and enable them to remain competitive in the global market. Our laboratory is focused on understanding the molecular basis of sperm function. The objective of this proposal is to investigate the potential of a testis-specific isozyme of Angiotensin Converting Enzyme (tACE; a sperm membrane protein) as a fertility marker in dairy bulls. We expect that this collaborative project with dairy industries will elucidate the role of tACE in bull fertility, train highly qualified individual for academia and industry, and facilitate development of novel laboratory assays for predicting fertility of frozen semen at their earliest possible age. Our long-term goal is to generate new knowledge regarding molecular regulation of sperm function and translate this knowledge to applications at the industry level for bull selection and evaluation of frozen semen.

即使牛的生殖率也适量提高，也可以显着提高生产率。因此，提高生殖效率的研究对乳制品行业很重要。在归类为满意的冷冻精液样本中，生育率有20-25％（基于传统的精液评估），这意味着精子的结构和功能在商业AI中心维持的精英公牛的结构和功能存在差异。了解精子受精能力中这些变化的分子基础将在乳制品行业中具有许多应用，因为它将使我们能够：1）将精子功能中的变异遗传基础纳入公牛选择模型中，以选择具有高生育能力的公牛的最早年龄； 2）确定用于调节调节生育能力的基因表达的牛犊管理的新策略（例如营养）； 3）确定生育能力的分子标记，这将使AI行业能够开发实验室测试，以精确地记录冷冻精液的生育能力，并使它们能够在全球市场保持竞争力。我们的实验室专注于理解精子功能的分子基础。该建议的目的是将血管紧张素转化酶（TACE；精子膜蛋白）作为乳制品公牛中的生育标记的血管紧张素转化酶（TACE；精子膜蛋白）的潜力。我们预计，这个与乳业行业的合作项目将阐明TACE在牛肥中的作用，训练高素质的学术界和工业，并促进开发新型实验室测定法，以预测最早可能的年龄冻结精液的生育能力。我们的长期目标是产生有关精子功能分子调节的新知识，并将这些知识转化为行业层面的应用，以进行牛市的选择和评估冷冻精液。