Effect of TASCO, a seaweed extract on rumen functions of ruminants: microbial diversity and activities in sheep

海藻提取物 TASCO 对反刍动物瘤胃功能的影响：绵羊微生物多样性和活性

• 批准号: 459358-2013
• 负责人: Guan, Leluo
• 金额: $ 2.55万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=576060
• 关键词: Effect; TASCO; seaweed; extract; rumen

Improvement of animal's production and health is vital to enhance the sustainability of Canadian livestock industry. The rumen is a capacious pre-gastric anaerobic fermentation chamber sustaining a rich community of microorganisms that rapidly colonize and digest feed particles. The rumen and its complex microbial community play a major role in providing energy in the form of volatile fatty acids and microbial protein for the metabolic function of the host. Therefore, improve the rumen function may play an essential role to significantly enhance the production efficiency of ruminants. In addition, recent outbreak of E. coli O157:H7 in a feedlot beef cattle processing plant in Alberta reveals the colonization of this pathogenic bacteria in GIT of animals and handling of the GIT parts of contaminated animals may be one of the courses to contaminate the plant. Therefore, reducing the colonization of this pathogenic bacterium in the GIT of cattle can significantly reduce the incidence of the contamination of this bacterium in the plant. Tasco®- AOS, is a natural product containing a mixture of naturally derived polysaccrides and oligosaccharides extracted from seaweed Ascophyllum nodosum by Acadian Seaplants Ltd., a Nova Scotia based Canadian company. Although Tasco®- AOS has been shown to have bioactive functions which improve the health of an animal's GI tract, its effects on the microbial ecology in ruminants has not been well characterized. Recent studies have revealed Tasco®- AOS can affect the activities of isolated rumen bacteria, as well as the in vitro ruminal digestion of different feeds and may reduce the shedding of E. coli O157:H7 in ruminants. These suggest that Tasco®- AOS may impact rumen function and potentially alter microbial populations through the exclusion of pathogens such as E. coli O157:H7. Therefore, the proposed project aims to investigate 1) whether Tasco®- AOS can improve ruminal microbial activity and rumen epithelial function, and 2) if this product can reduce the E. coli O157:H7 colonization of GIT of lambs.

提高动物的生产和健康水平对提高加拿大畜牧业的可持续性至关重要。瘤胃是一个宽敞的胃前厌氧发酵室，维持着丰富的微生物群落，这些微生物迅速定殖和消化饲料颗粒。瘤胃及其复杂的微生物群落以挥发性脂肪酸和微生物蛋白质的形式为宿主的代谢功能提供能量。因此，改善瘤胃功能对显著提高反刍动物的生产效率可能起着至关重要的作用。此外，最近爆发的E.阿尔伯塔肉牛养殖场的大肠杆菌O 157：H7的分离鉴定表明，该病原菌在动物GIT中定植，对受污染动物GIT部位的处理可能是污染植物的途径之一。因此，减少这种病原性细菌在牛胃肠道中的定殖可以显着降低该细菌在植物中污染的发生率。 Tasco®- AOS是一种天然产品，含有天然来源的多糖和寡糖的混合物，由Agodian Seaplants Ltd.一家位于加拿大新斯科舍省的公司。尽管Tasco®- AOS已被证明具有改善动物胃肠道健康的生物活性功能，但其对反刍动物微生物生态的影响尚未得到很好的表征。最近的研究表明，Tasco®- AOS可以影响瘤胃细菌的活性，以及不同饲料的体外瘤胃消化，并可能减少大肠杆菌的脱落。大肠杆菌O 157：H7。这表明Tasco®- AOS可能会影响瘤胃功能，并可能通过排除病原体（如大肠杆菌）来改变微生物种群。coli O157：H7。因此，本项目旨在研究1）Tasco®- AOS是否能够改善瘤胃微生物活性和瘤胃上皮功能，以及2）该产品是否能够降低E.大肠杆菌O 157：H7在羔羊胃肠道的定植。