"Microplate reader, a necessary tool for functional genomics and proteomics studies in animal, microbial and plant systems"

“酶标仪，动物、微生物和植物系统功能基因组学和蛋白质组学研究的必要工具”

• 批准号: 422258-2012
• 负责人: Guan, Leluo
• 金额: $ 2.82万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=495757
• 关键词: Microplate; reader; necessary; tool; functional

High throughput functional genomics and proteomics studies are the major focus of the research conducted in a multi-user Agricultural Genomics and Proteomics Unit (AGP Unit) in the Department of Agricultural, Food and Nutritional Science. Various researchers from The Livestock Genomics Group, The Dairy Nutritional Research Group, The Swine Reproduction-Development Program, Plant Biotechnology and Plant pathology group, Canola Breeding Program, Human Nutrition program, Poultry Research Group, Immunology, Food microbiology, Functional Foods and Nutraceuticals group, and collaborating scientists at Agriculture and Agri-Food Canada are currently supporting the research programs of over 100 users. All these groups are applying innovative efforts to improve production and reproductive performance of livestock, developing animal products with improved nutritional qualities, understanding molecular mechanisms of host-microbial interactions and creating crops with improved disease resistance and value added characteristics. All these applicants require the microplate reader for a wide variety of applications including reporter gene assays, DNA/RNA/protein quantitation and purity, PicoGreen/Bradford assay, cell applications, genotyping, enzyme assays, receptor ligand binding, immunoassays, screening for transgenics and mutants lines, and quantification of bioactive peptides and metabolites for phenotypic characterization. Acquisition of the plate reader with capabilities including luminescence, fluorescence Intensity (both top and bottom reading), UV-absorbance (UV-VIS) and time-resolved fluorescence is very critical for the progress of the research of all the major users as well as additional minor users within this large core facility. The research supported by this infrastructure will play an important role towards the future sustainability and profitability of Canadian agriculture industries, and training highly qualified personnel.

高通量功能基因组学和蛋白质组学研究是农业、食品和营养科学系多用户农业基因组学和蛋白质组学单位（AGP单位）进行的研究的主要焦点。来自家畜基因组学组、乳制品营养研究组、猪繁殖发展计划、植物生物技术和植物病理学组、油菜育种计划、人类营养计划、家禽研究组、免疫学、食品微生物学、功能性食品和营养保健品组的各种研究人员以及加拿大农业和农业食品部的合作科学家目前正在支持超过 100 个用户。所有这些团体都在采取创新措施来提高牲畜的生产和繁殖性能，开发具有改善营养品质的动物产品，了解宿主-微生物相互作用的分子机制，并创造具有更高抗病性和增值特性的作物。所有这些申请人都需要酶标仪用于各种应用，包括报告基因测定、DNA/RNA/蛋白质定量和纯度、PicoGreen/Bradford 测定、细胞应用、基因分型、酶测定、受体配体结合、免疫测定、转基因和突变体系筛选以及生物活性肽和代谢物的定量以进行表型表征。获得具有发光、荧光强度（顶部和底部读数）、紫外吸光度 (UV-VIS) 和时间分辨荧光等功能的读板器对于这个大型核心设施中所有主要用户以及其他次要用户的研究进展非常关键。该基础设施支持的研究将对加拿大农业产业未来的可持续性和盈利能力以及培养高素质人才发挥重要作用。