Lensless microscopy for rapid portable hematology diagnostics at the point of care

无透镜显微镜可在护理点进行快速便携式血液学诊断

• 批准号: 478477-2015
• 负责人: Fine, Alan
• 金额: $ 10.49万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Collaborative Health Research Projects
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=579542
• 关键词: Lensless; microscopy; rapid; portable; hematology

We are developing a new lensless imaging technology for pointof-care diagnostic testing. This technology enables us to create pocket-sized diagnostic devices with greater resolution but far lower cost than ordinary lens-based microscopes or other instruments for corresponding diagnostic use. These devices require only a drop of blood from a pinprick, rather than tubes of blood from a vein, and will yield immediate results in the patients presence. We will use this technology to develop rapid blood cell diagnostics, focusing on complete blood counts (which are among the most commonly ordered of all diagnostic blood tests), as well as detection of abnormal cells (e.g., sickle cells) and absolute counts of specific lymphocyte types, e.g., CD4+ cells (a key marker of immune system function in HIV disease). Starting from our current proof-of-principle prototypes, the devices will be optimized for these tests, including specimen handling and computer vision software for automated quantitative analysis. Devices will be validated by direct comparison (including in clinical settings) with gold standard tests in current hospital use. These new devices will be suitable for use in hospitals, clinics, physicians offices, emergency rooms and, uniquely, in remote settings: devices will be battery powered and Bluetooth-enabled, so data can be relayed, if needed, to specialists at distant sites.

我们正在开发一种新的无透镜成像技术，用于定点诊断测试。这项技术使我们能够 创造口袋大小的诊断设备，具有更高的分辨率，但成本远低于普通的透镜显微镜， 用于相应诊断用途的其他仪器。这些设备只需要从针刺一滴血，而不是 从静脉中抽取血液，并在患者在场时立即产生结果。我们将利用这项技术来开发 快速血细胞诊断，重点是全血细胞计数（这是最常见的命令之一， 诊断血液测试），以及异常细胞的检测（例如，镰状细胞）和特定淋巴细胞的绝对计数 类型，例如，CD4+细胞（HIV疾病中免疫系统功能的关键标志物）。从我们目前的原理证明开始 这些设备将针对这些测试进行优化，包括样本处理和计算机视觉软件， 自动定量分析。将通过与黄金直接比较（包括在临床环境中）来确认器械 目前医院使用的标准测试。 这些新设备将适用于医院、诊所、医生办公室、急诊室， 远程设置：设备将由电池供电并启用蓝牙，因此如果需要，可以将数据转发给 遥远的地方。