Optimization of sampling, detection and characterization of subsurface microbial life in systems with low permeability and low biomass-sedimentary and crystalline settings

低渗透性和低生物量沉积和结晶环境系统中地下微生物生命的采样、检测和表征的优化

• 批准号: 484516-2015
• 负责人: SherwoodLollar, Barbara
• 金额: $ 10.66万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=584374
• 关键词: Optimization; sampling; detection; characterization; subsurface

A key component in demonstrating the safety of a deep geologically isolated repositories (Deep Geologic Repositories: DGR) is the demonstration that microorganisms indigenous to the geologic system do not pose a concern to the integrity of the repository and thus to the local environment, including groundwater and surface-based ecosystems. Recent research demonstrates that microbial life inhabits the Earth's lithosphere to a far greater extent than was previously thought. While these organisms may be present in situ at very low abundances and/or in sessile or sporulated states, changing conditions over time could result in stimulation of in situ microbial activity. In order to predict and manage the potential for such impacts during planning and construction of a Canadian DGR, it is necessary to develop methods that can detect and identify microorganisms living within geologic systems with the greatest possible sensitivity. In low permeability rock with low microbial biomass and activity, the potential for false negatives is very high. This research proposal will develop the sampling approaches and innovative analytical approaches and methodology to identify and quantify microbial biomass and to assess the biomarkers of microbial activity in low permeability continental subsurface systems. The insights developed in this research will enable NWMO to undertake effective planning and construction activities for DGRs in Canada, and to inform future site characterization and selection. In addition, these findings will further expand our understanding of the limits, capability and survival of subsurface life on Earth. In addition to the knowledge benefits this proposal will train HQP in expertise relevant to ongoing implementation and improvement of scientific knowledge to support and improve Canada's energy and environmental sectors.

证明深层地质隔离储存库（深层地质储存库：DGR）安全性的一个关键组成部分是证明地质系统固有的微生物不会对储存库的完整性造成影响，从而不会对当地环境（包括地下水和地表生态系统）造成影响。 最近的研究表明，微生物生命栖息在地球岩石圈中的程度比之前想象的要大得多。虽然这些生物体可能以非常低的丰度和/或以无柄或孢子状态存在于原位，但随着时间的推移，条件的变化可能会导致原位微生物活动的刺激。为了在加拿大 DGR 的规划和建设过程中预测和管理此类影响的可能性，有必要开发能够以尽可能高的灵敏度检测和识别地质系统中生活的微生物的方法。 在微生物生物量和活性较低的低渗透性岩石中，出现假阴性的可能性非常高。该研究提案将开发采样方法和创新的分析方法和方法，以识别和量化微生物生物量并评估低渗透性大陆地下系统中微生物活动的生物标志物。这项研究中得出的见解将使 NWMO 能够对加拿大的 DGR 进行有效的规划和建设活动，并为未来的场地特征和选择提供信息。此外，这些发现将进一步扩大我们对地球地下生命的限制、能力和生存的理解。除了知识效益外，该提案还将培训总部专业知识，使其掌握与持续实施和改进科学知识相关的专业知识，以支持和改善加拿大的能源和环境部门。