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Aerobic Type II Methanotrophic Bacteria as a Biotechnological Platform for the Bioconversion of Methane to Succinic Acid

需氧 II 型甲烷氧化细菌作为甲烷生物转化为琥珀酸的生物技术平台

基本信息

批准号：
486083-2015
负责人：
Constant, Philippe
金额：
$ 1.82万
依托单位：
Institut national de la recherche scientifique
依托单位国家：
加拿大
项目类别：
Engage Grants Program
财政年份：
2015
资助国家：
加拿大
起止时间：
2015-01-01 至 2016-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=585387
关键词：
Aerobic Type II Methanotrophic Bacteria

项目摘要

In response to political and public pressure to mitigate greenhouse gas emissions, petroleum derived chemicals are increasingly substituted by more sustainable products. For instance, succinic acid is emerging as a competitive new generation bio-based building blocks in green chemistry to make a broad range of home products including adhesives and biodegradable plastics. Bioamber Inc. uses renewable feedstock such as non-food biomass and industrial waste to produce bio-based succinic acid through fermentation. Being the main component of natural gas (typically 90% v/v) and constituting 50% biogas generated in landfill, methane (CH4) is considered as the next generation carbon feedstock. To avail on such opportunity and combine beneficial greenhouse gas emission abatement with substitution of petroleum in the production of chemicals, the R&D team of the company is interested to explore the bioconversion of CH4 into succinic acid. In this project, aerobic bacteria using CH4 as sole energy and carbon sources will be tested for their ability to tolerate and grow in the presence of succinic acid. Selected bacteria available in public culture collections and new enrichment cultures will be tested. Succinic acid tolerant, fast-growing isolates reaching high cell density will be of special interest for downstream metabolic engineering efforts, leading to large-scale industrial applications. The proof of concept of this research project, if successful, will contribute to develop new green chemistry applications reducing our greenhouse gas emissions thus contributing to enhanced sustainability and transitioning, as well, towards a clean CH4-based biotechnology applicable worldwide.

为了应对减少温室气体排放的政治和公众压力，石油衍生化学品越来越多地被更可持续的产品所取代。例如，琥珀酸正在成为一种具有竞争力的新一代生物基积木在绿色化学，使广泛的家庭产品包括粘合剂和生物降解塑料。生物琥珀公司使用可再生原料，非食品生物质和工业废弃物，通过发酵生产生物基琥珀酸。作为天然气的主要成分（通常为90%v/v），占填埋场产生的沼气的50%，甲烷 (CH4)被认为是下一代碳原料。利用这样的机会和联合收割机在化学品生产中替代石油，有利于减少温室气体排放，该公司的研发团队有兴趣探索甲烷生物转化为琥珀酸。在这项目，将测试以CH 4为唯一能源和碳源的好氧细菌的耐受能力，并在琥珀酸存在下生长。公共培养物收藏中的选定细菌和新的将测试增菌培养物。琥珀酸耐受性，快速生长的菌株达到高细胞密度，特别感兴趣的下游代谢工程的努力，导致大规模的工业应用.本研究项目的概念验证如果成功，将有助于开发新的绿色化学应用减少我们的温室气体排放，从而有助于增强可持续性，此外，还向适用于全世界的清洁的以甲烷为基础的生物技术过渡。