Identification of predictive microRNA biomarkers for improved success of assisted reproductive technologies

鉴定预测性 microRNA 生物标志物以提高辅助生殖技术的成功率

• 批准号: 486352-2015
• 负责人: LaMarre, Jonathan
• 金额: $ 1.81万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=585537
• 关键词: Identification; predictive; microRNA; biomarkers; improved

Assisted reproductive technologies (ART) encompass evidence-based approaches to reproduction that lead to improved fertility through the integration of pharmacological, clinical and laboratory procedures. Growth in the requirement for improved fertility outcomes has lead to the emergence of clinic-based industries in both the medical and veterinary sectors that are dedicated to enhancing the success of ART procedures such as in vitro fertilization (IVF) and embryo transfer (ET). These procedures involve the acquisition of oocytes (eggs) from the mother, fertilizing them with sperm from the father, and incubating them through the earliest phases of development prior to reintroduction into the mother. With this proposal, we aim to initiate a partnership with ONE Fertility, an industry-leading Ontario Fertility clinic, to address an important problem affecting the entire industry, namely, how to identify embryos with the highest probability of a successful pregnancy. Embryos from human ART clinics cannot be readily used to develop improvements on existing technologies as any subsequent pregnancy might be jeopardized. Furthermore, many of the "models" such as mice that are often used in medical research are markedly different from humans. In contrast, the developmental events are highly similar between humans and cattle, and we routinely employ similar IVF protocols for this species. Importantly, preliminary studies have shown that small specific RNA markers are critical for development in both human and bovine embryos and have the potential to reflect the likelihood of a successful pregnancy. Using bovine embryos, we will examine several such markers that are also present in humans to determine how they change and whether the changes are associated with successful embryo growth. We will also determine whether the levels change in the media in which the embryos are grown. If successful, this project will establish important principles and a key model that can be employed by the ART industry to improve reproductive success.

辅助生殖技术(ART)包括以证据为基础的生殖方法，导致 通过整合药理、临床和实验室程序提高生育率。全球经济增长 对改善生育结果的要求导致了两个以临床为基础的行业的出现 医疗和兽医部门致力于提高体外抗逆转录病毒治疗程序的成功 受精(IVF)和胚胎移植(ET)。这些程序包括获取卵母细胞(卵)。 母亲，用父亲的精子使他们受精，并通过 在重新进入母体之前的发育。通过这项提议，我们的目标是启动与 One Fertivity，一家行业领先的安大略省生育诊所，解决影响整个 行业，即如何识别成功怀孕概率最高的胚胎。 来自人类ART诊所的胚胎不能轻易地用来改进现有的 技术，因为任何后续怀孕都可能受到威胁。此外，许多“模特”，如 经常用于医学研究的小鼠与人类有明显的不同。相比之下， 人类和牛的发育过程非常相似，我们经常使用类似的试管受精 为这个物种制定的协议。重要的是，初步研究表明，小的特异性RNA标记是 对人类和牛胚胎的发育至关重要，并有可能反映出 成功怀孕。使用牛胚胎，我们将检查几个这样的标记，这些标记也存在于 以确定它们是如何变化的，以及这些变化是否与成功的胚胎生长有关。 我们还将确定胚胎生长所在的培养液中的水平是否发生变化。如果成功， 该项目将确立重要的原则和关键模式，可供艺术行业采用 提高生育成功率。