Improving biopolymer yield in the feedstock plant Camelina sativa through hybridization

通过杂交提高原料植物亚麻荠的生物聚合物产量

• 批准号: 487019-2015
• 负责人: Schoen, Daniel
• 金额: $ 1.81万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=585895
• 关键词: Improving; biopolymer; yield; feedstock; plant

The manufacture of plastics has negative consequences for environmental health. The replacement of petroleum-based feedstocks with biomass as the source of chemical intermediates and recyclable biopolymers is one solution to this problem. Transformation of plants in the Brassicaceae family with genes from the bacterial species Alcaligenes eutrophus enables plants to synthesize complex organic compounds that possess the properties of biodegradable plastics normally derived from petroleum. To achieve high yields of these compounds, they must be produced in hybrid seed, as when present in vegetative tissue, they lead to poor plant growth. Pollination control, and hybridization is thus a major requirement for exploiting plant-based feedstocks for bioplastic production. We propose to develop a new tool, "Leavenworthia self-incompatibility" (LSI), for pollination control in Brassicaceae feedstock species where this capability is presently absent (e.g., Camelina sativa). This model plant is one of the most promising Canadian renewable feedstocks, and has been the subject of a recent $4.5 M Genome Prairie sequencing effort. This plant is undergoing development for biopolymer production by the Canadian company, Metabolix Oilseeds (Saskatoon). By genetically transforming this species with SI genes from the related plant species, Leavenworthia alabamica, we propose to gain improved understanding of the expression and activity of LSI genes in Camelina, as required for an effective system of enforcement of hybridization in the biopolymer production process developed by our partner, Metabolix Oilseeds. The company has thus far been unable to solve this problem in Camelina. Developing an improved understanding of the underlying molecular biology of this new and promising hybridization system will allow us to contribute to the advancement of fundamental knowledge that will facilitate the transition to sustainable and biodegradable sources of petrochemical substitutes for Canada's green economy.

塑料的制造对环境健康产生负面影响。的替换 以石油为基础的原料，以生物质为化学中间体和可回收生物聚合物的来源 是解决这个问题的一种方法。用来自 的基因转化十字花科植物 细菌种类真养产碱菌使植物能够合成具有以下特性的复杂有机化合物： 通常从石油中提取的可生物降解塑料的特性。为了实现这些项目的高产 化合物，它们必须在杂交种子中产生，因为当存在于营养组织中时，它们会导致植物生长不良 生长。因此，授粉控制和杂交是开发基于植物的主要要求 生物塑料生产的原料。我们建议开发一种新工具“Levenworthia自交不亲和性” (LSI)，用于十字花科原料物种的授粉控制，但目前尚不具备这种能力（例如， 亚麻荠)。该模型工厂是加拿大最有前途的可再生原料之一，并且已被 最近耗资 450 万美元的 Genome Prairie 测序工作的主题。该工厂正在开发中 生物聚合物由加拿大公司 Metabolix Oilseeds（萨斯卡通）生产。通过基因 用来自相关植物物种Leavenworthia alabamica的SI基因转化该物种，我们建议 更好地了解亚麻荠中 LSI 基因的表达和活性，以满足 我们开发的生物聚合物生产过程中实施杂交的有效系统 合作伙伴，Metabolix 油籽。迄今为止，该公司还无法解决卡梅利纳的这一问题。 更好地了解这种新的、有前途的分子生物学的基础 杂交系统将使我们能够为基础知识的进步做出贡献，这将 促进加拿大的石化替代品向可持续和可生物降解的来源过渡 绿色经济。