In Situ Biodegradation Potential in Petroleum Hydrocarbon-Contaminated Soils Exposed to Seasonal Freeze-Thaw Conditions.

暴露于季节性冻融条件下的石油烃污染土壤的原位生物降解潜力。

• 批准号: 488995-2015
• 负责人: Chang, WonJae
• 金额: $ 1.82万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=586432
• 关键词: Situ; Biodegradation; Potential; Petroleum; Hydrocarbon

Petroleum hydrocarbons are non-aqueous phase liquids (NAPLs) that include carcinogenic and toxic organic compounds, and are a cause for concern in soil environments impacted by oil extraction and transportation, accidental oil spills, and the disposal of oil sands wastes. According to the Federal Contaminated Sites Inventory, the most frequently identified contaminants in Canada are petroleum hydrocarbons, and many contaminated sites in Canada are influenced by cold-climate seasonality. Stimulating indigenous cold-adapted bacteria to degrade hydrocarbons has been considered a feasible, less destructive, and low-cost remedial option for hydrocarbon-contaminated soils at summer temperatures in cold climates. However, soils in cold regions remain partially frozen and frozen for 6 to 9 months each year, which greatly shortens the active remediation period, and current remediation practices in cold climates have only been considered effective during short summers (2-4 months), mainly due to colder temperatures and limited site accessibility during non-summer months. Dr. Won Jae Chang's recent study, however, indicated a significant potential for microbial enhancement in hydrocarbon-contaminated soils under seasonal freeze-thaw conditions during pre- and post-summers in cold climates. This new finding provides the rationale for this proposed pilot-scale biopile field study entitled In Situ Biodegradation Potential in Petroleum Hydrocarbon-Contaminated Soils Exposed to Seasonal Freeze-Thaw Conditions. There is a lack of field data about the extent of hydrocarbon biodegradation and microbial response in contaminated soils subjected to natural seasonal freeze-thaw temperatures. Changes in soil temperatures and unfrozen soil water during seasonal freeze-thaw periods are much slower than the rates used in many previous laboratory freeze-thaw studies, and provide the real conditions needed for native hydrocarbon-degrading bacteria to survive and/or adapt to environmental stresses in cold soils. The proposed study will generate field data by monitoring microbial activity under natural temperature cycles and will therefore provide new insight for bioremediation in environmentally challenging conditions.

石油烃是非水相液体（NAPL），其包括致癌和有毒的有机物 化合物，并且是受石油开采和运输影响的土壤环境中令人担忧的原因， 意外漏油和油砂废料的处理。根据联邦污染场地 在加拿大，最常见的污染物是石油烃， 加拿大受污染的场地受到寒冷气候季节性的影响。刺激当地适应寒冷的 细菌降解碳氢化合物被认为是一种可行的、破坏性较小的、低成本的补救选择 在寒冷的夏季温度下，碳氢化合物污染的土壤。然而，寒冷地区的土壤 每年保持部分冻结和冻结6至9个月，大大缩短了主动修复的时间 目前在寒冷气候中的补救措施只在短期内被认为是有效的。 夏季（2-4个月），主要是由于非夏季气温较低，场地可达性有限 个月然而，Won Jae Chang博士最近的研究表明， 在季节性冻融条件下， 在寒冷的气候中度过夏天。这一新发现为这一拟议的中试规模生物池提供了理论基础 题为“石油烃污染土壤暴露于环境中的就地生物降解潜力”的实地研究 季节性冻融条件。缺乏关于碳氢化合物生物降解程度的现场数据 以及受污染土壤在自然季节性冻融温度下的微生物反应。变化 在季节性冻融期间，土壤温度和未冻土壤水分的变化速度要比 在许多以前的实验室冻融研究中使用，并提供了天然冻融所需的真实的条件。 烃降解细菌在寒冷的土壤中生存和/或适应环境压力。拟议 这项研究将通过监测自然温度循环下的微生物活动来生成现场数据， 因此，为在环境挑战性条件下进行生物修复提供了新的见解。