Microbial community response to biostimulation in a petroleum hydrocarbon contaminated site in Saskatoon, SK

萨斯卡通石油碳氢化合物污染场地微生物群落对生物刺激的反应

• 批准号: 491896-2015
• 负责人: McBeth, Joyce
• 金额: $ 1.82万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=587129
• 关键词: Microbial; community; response; biostimulation; petroleum

We propose to characterize and develop a conceptual model for microbial community changes associated with biostimulation of a hydrocarbon contaminated aquifer in Saskatoon, SK. The aquifer was contaminated by a fuel station over a period of several decades, and is one of many similar sites managed by the industry partner on this project, Federated Cooperatives Limited (FCL). These sites present a long-term liability for FCL's member co-operatives, and they are keen to refine the approaches they use to remediate these sites. To achieve this objective, it is important to have a holistic understanding of the interplay between the local geology, the distribution of the contaminants, the microbiology of the aquifer groundwater and soils, and the impact of biostimulation amendments on the system through time. We will use high-throughput amplicon sequencing to characterize microbial community changes in the groundwater at the site over a 6 month biostimulation period. We will also characterize core samples taken from the site to examine the in-situ microbiology and mineralogy in the soils prior to biostimulation.These data, along with geochemical analyses provided by FCL, will be used to construct a conceptual model for the biogeochemical changes driven by biostimulation in the aquifer. This will help us to build our understanding of how microbes respond to biostimulation at this site and and improve the strategies FCL uses for remediation of this and similar sites moving forward.

我们建议描述和开发一个概念模型，用于描述与生物刺激相关的微生物群落变化，这些变化与萨斯卡通的碳氢化合物污染含水层有关。该含水层在几十年的时间里被一个加油站污染，是该项目的行业合作伙伴联合合作社有限公司(FCL)管理的许多类似地点之一。这些地点对FCL的成员合作社来说是一个长期的负担，他们热衷于改进他们用来补救这些地点的方法。为了实现这一目标，重要的是全面了解当地地质、污染物分布、含水层地下水和土壤的微生物以及随着时间的推移生物刺激修正对系统的影响。我们将使用高通量扩增子测序来表征在6个月的生物刺激期内现场地下水中微生物群落的变化。我们还将对从现场采集的岩心样本进行表征，以检查生物刺激之前土壤中的原位微生物和矿物学。这些数据将与FCL提供的地球化学分析一起用于构建含水层生物刺激驱动的生物地球化学变化的概念模型。这将有助于我们建立我们对微生物如何对该地点的生物刺激做出反应的理解，并改进FCL用于修复该地点和类似地点的策略。