Synthesis and function of novel bacterial carbohydrates

新型细菌碳水化合物的合成与功能

• 批准号: RGPIN-2015-05717
• 负责人: Creuzenet, Carole
• 金额: $ 2.48万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=590596
• 关键词: Synthesis; function; novel; bacterial; carbohydrates

The glycome represents an extra layer of information above the genome, transcriptome and proteome and deciphering its role is a fundamental question. This is very difficult for bacterial carbohydrates that are highly variable. Elucidating their biosynthesis will allow assessing their function and harnessing the potential of novel enzymes for biotechnology applications. We have focused on modified heptoses from Campylobacters or Yersiniae and characterized 9 heptose modification enzymes that affect the function of the capsule and lipopolysaccharide in which the modified heptoses reside, and that promote chicken colonization by

糖组代表了基因组、转录组和蛋白质组之上的额外信息层，破译其作用是一个基本问题。这对于高度可变的细菌碳水化合物是非常困难的。阐明它们的生物合成将允许评估它们的功能并利用新酶的生物技术应用潜力。我们集中于来自弯曲杆菌或耶尔森氏菌的修饰的庚糖，并表征了9种庚糖修饰酶，其影响修饰的庚糖所在的荚膜和脂多糖的功能，并通过以下方式促进鸡定殖：