Critical Addition of a Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) Mass Spectrometer for the University of Waterloo Mass Spectrometry Facility

滑铁卢大学质谱设施关键添加基质辅助激光解吸/电离飞行时间 (MALDI-TOF) 质谱仪

• 批准号: RTI-2016-00008
• 负责人: Gauthier, Mario
• 金额: $ 10.93万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=591305
• 关键词: Critical; Addition; Matrix; Assisted; Laser

Critical requirements for the successful mass spectrometric analysis of high molar mass (bio)materials/(bio)polymers are a high sensitivity and the ability to measure the molar mass accurately to > 100 kDa. The University of Waterloo Mass Spectrometry Facility (UWMSF) has state-of-the-art systems for the analysis of small molecules by electrospray ionization (ESI), atmospheric pressure chemical ionization, direct analysis in real time, and ultra-high performance liquid chromatography coupled to mass spectrometry and tandem mass spectrometry. However these capabilities cannot address demands to characterize nonvolatile and high molar mass materials resulting in many research programs being severely curtailed. We have investigated the usefulness of electrospray ionization (ESI) for these systems with very limited or no success. This critical deficiency can be addressed with the acquisition of a Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) instrument. No such equipment exists at the UW at present.

高摩尔质量（生物）材料/（生物）聚合物的成功质谱分析的关键要求是高灵敏度和精确测量摩尔质量至> 100 kDa的能力。滑铁卢大学质谱设施（UWMSF）拥有最先进的系统，可通过电喷雾电离（ESI）、大气压化学电离、真实的时间直接分析以及超高效液相色谱联用质谱和串联质谱分析小分子。然而，这些能力不能满足要求，以表征非挥发性和高摩尔质量的材料，导致许多研究计划被严重削减。我们已经研究了电喷雾离子化（ESI）对这些系统的有用性，但非常有限或没有成功。这一关键缺陷可以通过获得基质辅助激光解吸电离飞行时间（MALDI-TOF）仪器来解决。目前，UW还没有这样的设备。