Experimental evolution of phosphotriestrase to switch the catalytic activities

磷酸三酯酶转换催化活性的实验进化

• 批准号: 418262-2012
• 负责人: Tokuriki, Nobuhiko
• 金额: $ 2.26万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=596277
• 关键词: Experimental; evolution; phosphotriestrase; switch; catalytic

Evolution provides the theoretical framework by which we can understand biology. Understanding evolutionary dynamics is also crucial to develop technologies and methodologies to engineer and design new enzymes in the laboratory. Insights that have come from existing sequences have substantially developed our knowledge of molecular evolution. However, this traditional analysis does not examine evolutionary intermediates. It therefore limits our ability to investigate evolutionary mechanisms in detail. Experimental evolution enables us to reconstruct evolutionary processes in a controlled laboratory setting. The evolutionary intermediates obtained in the experiments represent a molecular "fossil record", and allow us to explore the step-by-step evolution of molecular function, and to elucidate how proteins have acquired diverse functions and structures.

进化论提供了我们理解生物学的理论框架。了解进化动力学对于开发技术和方法在实验室中设计和设计新酶也至关重要。来自现有序列的见解极大地发展了我们对分子进化的认识。然而，这种传统的分析并没有考察进化中间体。因此，它限制了我们详细研究进化机制的能力。实验进化使我们能够在受控的实验室环境中重建进化过程。实验中获得的进化中间体代表了分子的“化石记录”，使我们能够探索分子功能的逐步进化，并阐明蛋白质如何获得不同的功能和结构。