Development of paper-based immunoassays to detect food chemical hazards

开发纸基免疫测定法来检测食品化学危害

• 批准号: 486586-2015
• 负责人: Lu, Xiaonan
• 金额: $ 5.2万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=600313
• 关键词: Development; paper; based; immunoassays; detect

Rapid, cost-effective, and accurate detection of food chemical hazards can offer an important means to improve food safety and economic benefit of biotechnology industry in Canada. Enzyme-linked immunosorbent assay (ELISA) and immunochromatographic strip assay are two representative immunoassays that have been widely applied for the detection of food chemical hazards. However, conventional ELISA requires large volumes of analytes and reagents, and the time required for incubation and blocking steps are long. Further, the fabrication of immunochromatographic strip is complicated and the price of a single test is high. In this NSERC CRD project, we propose to develop low-cost paper-based immunoassays to improve the detection of food chemical hazards. As two representative techniques to pattern paper, photolithography and wax screen printing will be applied to fabricate microzone on paper for the development of both competitive and sandwich ELISA assays to detect selective veterinary drug residues and allergens, respectively. In addition, inkjet printing technique will be applied to fabricate microfluidic paper-based immunochromatographic strips for the detection of the aforementioned food chemical hazards in a "one-step" procedure. The developed paper-based immunoassays for the detection of food chemical hazards offer a few major advantages over the conventional immunoassays. They are more rapid, high-throughput, and only require small amount of samples, reagents, and simple equipment. The availability of this technology will provide Canadian manufactures with unprecedented insight and advantages in the development of the next-generation immunoassays. Continuous information certifying the performance quality of advanced immunoassays will secure the premier position of Canadian agri-food diagnostic products in the world marketplace. Moreover, the knowledge developed in the areas of patterned paper along with microfluidic "lab-on-a-chip" technique represent an advance of fundamental importance to the field of analytical chemistry that applied in agricultural, food, and biotechnology industry.

快速、经济、准确地检测食品中的化学危害物是提高加拿大食品安全和生物技术产业经济效益的重要手段。酶联免疫吸附试验（ELISA）和免疫层析试纸条法是两种具有代表性的免疫检测方法，已广泛应用于食品化学危害的检测。然而，常规ELISA需要大量的分析物和试剂，并且孵育和封闭步骤所需的时间长。此外，免疫层析条的制造复杂，并且单次测试的价格高。在这个NSERC CRD项目中，我们建议开发低成本的纸基免疫测定法，以提高食品化学危害的检测。作为两种具有代表性的图案纸技术，光刻和蜡丝网印刷将被应用于在纸上制作微区，以分别用于检测选择性兽药残留和过敏原的竞争性和夹心ELISA检测。此外，喷墨打印技术将被应用于制造微流控纸基免疫层析条，用于在“一步”程序中检测上述食品化学危害。开发的纸基免疫检测食品化学品危害提供了一些主要的优势，比传统的免疫检测。它们更快速，高通量，只需要少量的样品，试剂和简单的设备。该技术的可用性将为加拿大制造商在开发下一代免疫测定方面提供前所未有的洞察力和优势。持续不断的信息证明先进的免疫测定的性能质量将确保加拿大农业食品诊断产品在世界市场上的首要地位。此外，图案纸沿着微流控“芯片实验室”技术领域的知识发展代表了应用于农业、食品和生物技术工业的分析化学领域的根本重要性的进步。