An ultrasound-based approach to control foam in bioreactors

基于超声波的生物反应器泡沫控制方法

• 批准号: 494818-2016
• 负责人: Pritzker, Mark
• 金额: $ 1.82万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=603297
• 关键词: ultrasound; based; approach; control; foam

Foaming in bioreactors is a widespread problem in the pharmaceutical industry that is currently addressed with chemical antifoam reagents. The main disadvantages of using such reagents are that they i) cause mass transfer limitations in upstream operations, ii) create the need for extra steps in the subsequent purification processes, iii) cause cell lysis which in turn triggers the release of proteins/enzymes that further exacerbate foaming and reduce catalytic activity and iv) render the fermentation system less homogeneous. Thus, the pharmaceutical industry has a large incentive to mitigate foaming by novel non-chemical means.

生物反应器中的起泡是制药行业中普遍存在的问题，目前使用化学消泡剂来解决这一问题。使用这种试剂的主要缺点是：1)在上游操作中造成传质限制，2)在后续的纯化过程中需要额外的步骤，3)导致细胞裂解，这反过来又触发蛋白质/酶的释放，从而进一步加剧泡沫并降低催化活性，以及4)使发酵系统不那么均匀。因此，制药业有很大的动机通过新的非化学手段来减少泡沫。