A novel process for the decarboxylation, selective extraction, and purification of cannabidiol (CBD) from hemp

BELEAVE公司计划生产高质量的药用植物提取物，以治疗患有各种健康问题的患者，包括疼痛、炎症和关节炎组织损失。基于植物的处方，甚至是从药用植物组织中选择性提取的提取物，含有多种药学相关的化合物，包括萜酚，其必须被提取、分离和鉴定以用于药物标准化，并评估临床应用。需要在烘箱中使大麻二酚（CBD）脱羧，通过饮用乙醇和水的选择性混合物提取化合物，并通过离子交换色谱法将CBD与其他大麻素分离。除非CBD可以被常规地提取、分离、鉴定并以精确的剂量进行评估，否则将药物活性归因于治疗特定疾病的特定化合物和生产有用的生物药物将是困难的。因此，迫切需要了解CBD的提取和分离，并建立一种新的和有效的策略，用于使用可饮用溶剂从植物组织中提取和分离萜烯酚化合物，以产生用于定量萜烯酚如大麻二酚（CBD）的纯化产物。CBD具有与一些其他关键萜酚类似的分子量，然而CBD具有两个暴露的羟基，其可用于通过极性溶剂之间的分配或阴离子交换色谱将其与其他萜酚分离。来自非精神活性的药用大麻品种的花组织的CBD将在烘箱中通过一系列温度脱羧，从大麻提取CBD的溶解度（和沉淀点）将用一系列水/乙醇级分测定，并且将检查通过强或弱离子交换和C18反相色谱分离的CBD保留，以开发用于大麻提取的CBD的方法。

BELEAVE Inc plans to produce high-quality medicinal plant extracts to treat patients suffering from a variety of health issues, including pain, inflammation and tissue loss in arthritis. Plant-based prescriptions, and even selective extracts from medicinal plant tissues, contain a diversity of pharmaceutically relevant compounds including terpenophenols that must be extracted, isolated and identified for drug standardization, and to evaluate clinical applications. There is a need to decarboxylate cannabidiol (CBD) in an oven, extract the compound by selective mixtures of potable ethanol and water, and to separate the CBD from other cannabinoids by ion exchange chromatography. Unless the CBD can be routinely extracted, isolated, identified, and evaluated at precise doses, attribution of medicinal activity to specific compounds in the treatment of particular diseases and the production of useful biopharmaceuticals will be difficult. Thus, there is an urgent need to understand the extraction and separation of CBD and establish a novel and effective strategy for the extraction and isolation of terpenophenol compounds from plant tissues using potable solvents to yield purified products for the quantification of terpenophenols such as cannabidiol (CBD). CBD has a similar molecular mass to that of some other key terpenophenols, however CBD has two exposed hydroxyl groups that might be exploited to separate it from other terpenophenols by partition between polar solvents or anion exchange chromatography. The CBD from floral tissues of non-psychoactive, medicinal hemp cultivars will be decarboxlyted in an oven by a range of temperatures, the solubility (and precipitation point) for the extraction of CBD from hemp will be determined with a range of water/ethanol fractions, and CBD retention by strong or weak ion exchange and C18 reversed phase chromatography for isolation will be examined to develop a