A novel process for the decarboxylation, selective extraction, and purification of cannabidiol (CBD) from hemp

BELEAVE Inc.计划生产高质量的药用植物提取物，以治疗患有各种健康问题的患者，包括关节炎中的疼痛、炎症和组织丢失。以植物为基础的处方，甚至是从药用植物组织中选择性提取的提取物，都含有多种与药学相关的化合物，包括必须提取、分离和鉴定的萜类苯酚，以便进行药物标准化和评估临床应用。需要在烤箱中对大麻二酚(CBD)进行脱羧化，通过饮用乙醇和水的选择性混合物来提取化合物，并通过离子交换层析将CBD与其他大麻类化合物分离。除非能够以精确的剂量对CBD进行常规提取、分离、鉴定和评估，否则在治疗特定疾病和生产有用的生物药物时，很难将药用活性归因于特定的化合物。因此，迫切需要了解CBD的提取和分离，并建立一种新的、有效的策略来从植物组织中提取和分离萜烯酚化合物，使用可饮用的溶剂来生产用于定量测定大麻二酚等萜类苯酚的纯化产品。CBD与其他一些关键的萜类酚具有相似的分子质量，但CBD有两个暴露的羟基，可以通过极性溶剂分配或阴离子交换层析将其与其他萜类酚分离。从非精神活性的药用大麻品种的花组织中提取的CBD将在一定的温度范围内在烘箱中脱羧，从大麻中提取CBD的溶解度(和沉淀点)将用一定范围的水/乙醇组分来确定，并将通过强或弱离子交换和C18反相色谱分离CBD来考察CBD的保留率。

BELEAVE Inc plans to produce high-quality medicinal plant extracts to treat patients suffering from a variety of health issues, including pain, inflammation and tissue loss in arthritis. Plant-based prescriptions, and even selective extracts from medicinal plant tissues, contain a diversity of pharmaceutically relevant compounds including terpenophenols that must be extracted, isolated and identified for drug standardization, and to evaluate clinical applications. There is a need to decarboxylate cannabidiol (CBD) in an oven, extract the compound by selective mixtures of potable ethanol and water, and to separate the CBD from other cannabinoids by ion exchange chromatography. Unless the CBD can be routinely extracted, isolated, identified, and evaluated at precise doses, attribution of medicinal activity to specific compounds in the treatment of particular diseases and the production of useful biopharmaceuticals will be difficult. Thus, there is an urgent need to understand the extraction and separation of CBD and establish a novel and effective strategy for the extraction and isolation of terpenophenol compounds from plant tissues using potable solvents to yield purified products for the quantification of terpenophenols such as cannabidiol (CBD). CBD has a similar molecular mass to that of some other key terpenophenols, however CBD has two exposed hydroxyl groups that might be exploited to separate it from other terpenophenols by partition between polar solvents or anion exchange chromatography. The CBD from floral tissues of non-psychoactive, medicinal hemp cultivars will be decarboxlyted in an oven by a range of temperatures, the solubility (and precipitation point) for the extraction of CBD from hemp will be determined with a range of water/ethanol fractions, and CBD retention by strong or weak ion exchange and C18 reversed phase chromatography for isolation will be examined to develop a