Identity of micro organisms and enzymes that bio-degrade the surfactants, required to disperse heavy petroleum oil, in a model environment

在模型环境中生物降解分散重石油所需的表面活性剂的微生物和酶的特性

• 批准号: 458937-2013
• 负责人: Marshall, John
• 金额: $ 1.82万
• 依托单位: Ryerson University
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=542086
• 关键词: Identity; micro; organisms; enzymes; bio

A biodegradable surfactant might be used to clean water or soil contaminated with oil. The problem Green On has raised in this research proposal is the need to understand the mechanisms by which its proprietary surfactant that contains amphipathic molecules, and charged chelating agents will degrade in the environment. Understanding whether and how the surfactant is biodegradable may have tremendous commercial value for Green On. Cultures of micro organisms from soil, water or other sources with the surfactant will be analyzed by GC-MS, GC-FID to determine the rate at which the product degrades in a model environment. Analyzing the bacteria or fungal micro organisms by LC-ESI-MS/MS will reveal the specific species and enzyme systems that may degrade the surfactant. There is good reason to support the feasibility of this approach. This research will produce analytical measurements of the rate of degradation of the oil dispersant product DXR in soil and water. The oil dispersant contains the polymeric detergents triton x-100, triton-45, tall oil, xylene sulfone, EDTA and other amphipathic molecules for which there is no general test. Instrumental analysis methods such as Gas Chromatography-Mass Spectrometry (GC-MS) and GC-FID (Flame Ionization Detection) and liquid chromatography and tandem mass spectrometry (LC-ESI-MS/MS) will have to be developed to quantify the amount of the DXR dispersant in the model environment over time. In some instances LC-ESI-MS/MS may be used to show agreement with GC techniques. The immediate research challenges will be to determine the working range of DXR concentrations required to disperse oil in a model environment and to test bacteria obtained from the soil, lakes or sewers to degrade DXR. The main technical challenge will be to measure the rate of degradation of the product in the presence of soil, water and micro-organisms within a model environment using Gas Chromatography-Mass Spectrometry (GC-MS) and GC-FID (Flame Ionization Detection). Characterizing the proteins of the bacteria species identified in the bacterial population derived from the surfactant will be a long term goal of this research project.

一种可生物降解的表面活性剂可用于清洁被油污染的水或土壤。Green On在这项研究计划中提出的问题是，需要了解其专有的表面活性剂（含有两亲分子）和带电螯合剂在环境中降解的机制。了解表面活性剂是否可生物降解以及如何生物降解对绿安公司来说可能具有巨大的商业价值。用表面活性剂对土壤、水或其他来源的微生物培养物进行GC-MS、GC-FID分析，以确定产品在模型环境中降解的速率。通过LC-ESI-MS/MS对细菌或真菌微生物进行分析，可以发现降解表面活性剂的特定种类和酶系统。有充分的理由支持这种方法的可行性。这项研究将产生石油分散剂产品DXR在土壤和水中降解率的分析测量结果。该油分散剂含有聚合洗涤剂triton x-100、triton-45、塔尔油、二甲苯砜、EDTA等无通用测试的两亲性分子。必须开发气相色谱-质谱（GC-MS）和GC-FID（火焰电离检测）以及液相色谱和串联质谱（LC-ESI-MS/MS）等仪器分析方法，以量化模型环境中DXR分散剂随时间的量。在某些情况下，LC-ESI-MS/MS可用于显示与GC技术的一致性。当前的研究挑战将是确定在模型环境中分散石油所需的DXR浓度的工作范围，并测试从土壤、湖泊或下水道中获得的细菌降解DXR的能力。主要的技术挑战将是使用气相色谱-质谱（GC-MS）和GC-FID（火焰电离检测）在模型环境中测量产品在土壤，水和微生物存在下的降解率。表征从表面活性剂衍生的细菌种群中鉴定出的细菌种类的蛋白质将是本研究项目的长期目标。