Combined Mass Spectrometric and Optical Spectroscopic Investigation of Biological Molecules in Controlled Micro-environments
受控微环境中生物分子的质谱和光学光谱联合研究
基本信息
- 批准号:RGPIN-2015-04599
- 负责人:
- 金额:$ 3.28万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Discovery Grants Program - Individual
- 财政年份:2016
- 资助国家:加拿大
- 起止时间:2016-01-01 至 2017-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The development and application of new tools and techniques to identify molecules and study their properties can be transformative, with long-term impact on fields ranging from chemistry and physics to medicine and biology. The development of such tools is the aim of research in the Jockusch laboratory. In particular, we focus on the development of new techniques that extend the uses of mass spectrometry (MS) in order to enhance the understanding of how biological molecules such as proteins are affected by their environment. This is important because proteins play a central role in biology. For example, proteins that are ‘misfolded’ have negative impacts on health, and are associated with many devastating diseases including Alzheimer’s and Parkinson’s diseases. However, proteins and other biomolecules do not generally act in isolation; their behavior is modulated by a complex web of molecular interactions. In order to dissect the complexity present in Nature, it is valuable to study simplified systems whose complexity can gradually be built up in a carefully controlled fashion. Thus, the Jockusch laboratory’s approach to understanding the properties of biomolecules is to study them in a highly-controlled environment: isolated inside a mass spectrometer. This enables the separation of intrinsic molecular properties from effects of the molecular environment. In subsequent investigations, pieces of the molecular environment such as water molecules, metal ion, or other binding partners will be added to form complexes and clusters, thus enabling discovery of how additional specific interactions modulate or control the molecular properties of interest.
The Jockusch laboratory has added a new dimension of information to tandem mass spectrometry analysis by interfacing MS with a flexible interface for gas-phase laser spectroscopic experiments. In particular, we are spearheading the use of fluorescence and fluorescence resonance energy transfer (FRET) techniques to probe conformation and other functional characteristics of gaseous biomolecular ions and their complexes. FRET is an example of a transformative technique, developed by physicists and subsequently adopted by biologists for the study of protein conformation and dynamics. Its use enabled the study of even single molecules in solution – an amazing advance from ensemble techniques commonly used. The development of gas-phase FRET promises to be equally transformative. Only two other laboratories world-wide have capabilities and expertise similar to the Jockusch lab (at Harvard, and ETH Zurich), having implemented the powerful combination of mass spectrometry and fluorescence techniques. Not only will the techniques and tools developed in the Jockusch lab push the boundaries of MS-enabled science, this cutting-edge research program provides an excellent training environment for the next generation of Canadian Scientists.
识别分子和研究其性质的新工具和技术的开发和应用可能具有变革性,对从化学和物理到医学和生物学的各个领域都会产生长期影响。开发这类工具是Jockusch实验室的研究目标。特别是,我们专注于新技术的开发,以扩展质谱学(MS)的使用,以增强对蛋白质等生物分子如何受到环境影响的理解。这一点很重要,因为蛋白质在生物学中扮演着核心角色。例如,错误折叠的蛋白质对健康有负面影响,并与包括阿尔茨海默氏症和帕金森氏症在内的许多毁灭性疾病有关。然而,蛋白质和其他生物分子通常不是孤立地发挥作用;它们的行为受到复杂的分子相互作用网络的调节。为了剖析自然界中存在的复杂性,研究简化的系统是有价值的,这些系统的复杂性可以以一种谨慎控制的方式逐渐建立起来。因此,Jockusch实验室了解生物分子特性的方法是在高度受控的环境中研究它们:隔离在质谱仪内。这使得从分子环境的影响中分离固有的分子特性成为可能。在随后的研究中,将添加分子环境的一部分,如水分子、金属离子或其他结合伙伴,形成络合物和簇合物,从而能够发现额外的特定相互作用如何调节或控制感兴趣的分子性质。
Jockusch实验室通过将MS与用于气相激光光谱实验的灵活接口相连接,为串联质谱分析增加了新的信息维度。特别是,我们正在率先使用荧光和荧光共振能量转移(FRET)技术来探测气态生物分子离子及其络合物的构象和其他功能特征。FRET是一种变革性技术的例子,由物理学家开发,随后被生物学家用于研究蛋白质构象和动力学。它的使用使得研究溶液中的单分子成为可能--与通常使用的系综技术相比,这是一个惊人的进步。气相FRET的发展也同样具有变革性。世界上只有另外两个实验室拥有与Jockusch实验室(哈佛大学和苏黎世理工大学)类似的能力和专业知识,实现了质谱学和荧光技术的强大组合。Jockusch实验室开发的技术和工具不仅将突破MS支持的科学的界限,而且这一尖端研究项目为下一代加拿大科学家提供了良好的培训环境。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Jockusch, Rebecca其他文献
Jockusch, Rebecca的其他文献
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{{ truncateString('Jockusch, Rebecca', 18)}}的其他基金
Enabling Technologies to Characterize Biomolecules and Fluorophores in Controlled Micro-environments using Mass Spectrometry
使用质谱技术来表征受控微环境中的生物分子和荧光团
- 批准号:
RGPIN-2020-05828 - 财政年份:2022
- 资助金额:
$ 3.28万 - 项目类别:
Discovery Grants Program - Individual
Enabling Technologies to Characterize Biomolecules and Fluorophores in Controlled Micro-environments using Mass Spectrometry
使用质谱技术来表征受控微环境中的生物分子和荧光团
- 批准号:
RGPIN-2020-05828 - 财政年份:2021
- 资助金额:
$ 3.28万 - 项目类别:
Discovery Grants Program - Individual
Enabling Technologies to Characterize Biomolecules and Fluorophores in Controlled Micro-environments using Mass Spectrometry
使用质谱技术来表征受控微环境中的生物分子和荧光团
- 批准号:
RGPIN-2020-05828 - 财政年份:2020
- 资助金额:
$ 3.28万 - 项目类别:
Discovery Grants Program - Individual
Combined Mass Spectrometric and Optical Spectroscopic Investigation of Biological Molecules in Controlled Micro-environments
受控微环境中生物分子的质谱和光学光谱联合研究
- 批准号:
RGPIN-2015-04599 - 财政年份:2019
- 资助金额:
$ 3.28万 - 项目类别:
Discovery Grants Program - Individual
Combined Mass Spectrometric and Optical Spectroscopic Investigation of Biological Molecules in Controlled Micro-environments
受控微环境中生物分子的质谱和光学光谱联合研究
- 批准号:
RGPIN-2015-04599 - 财政年份:2018
- 资助金额:
$ 3.28万 - 项目类别:
Discovery Grants Program - Individual
Combined Mass Spectrometric and Optical Spectroscopic Investigation of Biological Molecules in Controlled Micro-environments
受控微环境中生物分子的质谱和光学光谱联合研究
- 批准号:
RGPIN-2015-04599 - 财政年份:2017
- 资助金额:
$ 3.28万 - 项目类别:
Discovery Grants Program - Individual
Combined Mass Spectrometric and Optical Spectroscopic Investigation of Biological Molecules in Controlled Micro-environments
受控微环境中生物分子的质谱和光学光谱联合研究
- 批准号:
477790-2015 - 财政年份:2017
- 资助金额:
$ 3.28万 - 项目类别:
Discovery Grants Program - Accelerator Supplements
Combined Mass Spectrometric and Optical Spectroscopic Investigation of Biological Molecules in Controlled Micro-environments
受控微环境中生物分子的质谱和光学光谱联合研究
- 批准号:
477790-2015 - 财政年份:2016
- 资助金额:
$ 3.28万 - 项目类别:
Discovery Grants Program - Accelerator Supplements
Combined Mass Spectrometric and Optical Spectroscopic Investigation of Biological Molecules in Controlled Micro-environments
受控微环境中生物分子的质谱和光学光谱联合研究
- 批准号:
477790-2015 - 财政年份:2015
- 资助金额:
$ 3.28万 - 项目类别:
Discovery Grants Program - Accelerator Supplements
Biophysical Analytical Chemistry
生物物理分析化学
- 批准号:
1220446-2010 - 财政年份:2015
- 资助金额:
$ 3.28万 - 项目类别:
Canada Research Chairs
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