UPLC System for Muscle Health Research

用于肌肉健康研究的 UPLC 系统

• 批准号: RTI-2017-00404
• 负责人: Hood, David
• 金额: $ 10.48万
• 依托单位: York University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=616667
• 关键词: UPLC; System; Muscle; Health; Research

Ultra High-performance Liquid Chromatography (UPLC) is a method which allows the researcher to separate, identify and quantify the components of a biological sample with great sensitivity. It relies on the use of pressure to pass the biological sample solution through a column. This solution can interact with materials present in this column and effectively separate molecules from each other, to help identify them. At York University we have a large number of researchers in the Department of Biology, the School of Kinesiology and Health Science, and the Muscle Health Research Centre (MHRC) that require the use of UPLC technology. In this application we are requesting funds to acquire only the second such equipment system on the York campus. We wish to use this to analyze amino acids, catecholamines, metabolites and peptides in biological samples such a serum, muscle, and heart primarily to support the research of MHRC members, but also our colleagues in other departments. My 5 co-applicants and I will make heavy use of this equipment, and as the Director of the MHRC, I am submitting this application also on behalf of the numerous (>30) other faculty members in the 3 research units above who do cellular biochemistry research and could potentially make use of this equipment. This equipment will increase our capacity for the fundamental biochemical analyses of complex biological samples, and its acquisition markedly help to dissipate the logjam of users who currently travel to another building to use the one other functional instrument on the York University campus.

超高效液相色谱 (UPLC) 是一种允许研究人员以极高的灵敏度分离、识别和定量生物样品成分的方法。它依靠压力使生物样品溶液通过色谱柱。该溶液可以与该色谱柱中的材料相互作用，并有效地将分子彼此分离，以帮助识别它们。在约克大学，我们的生物系、运动机能与健康科学学院以及肌肉健康研究中心（MHRC）有大量的研究人员需要使用 UPLC 技术。在此申请中，我们请求资金购买约克校区的第二个此类设备系统。我们希望用它来分析血清、肌肉和心脏等生物样品中的氨基酸、儿茶酚胺、代谢物和肽，主要是为了支持 MHRC 成员以及我们其他部门同事的研究。我和我的 5 名共同申请人将大量使用该设备，作为 MHRC 主任，我还代表上述 3 个研究单位中从事细胞生物化学研究并可能使用该设备的众多（> 30 名）其他教员提交此申请。该设备将提高我们对复杂生物样本进行基本生化分析的能力，它的获得显着有助于消除目前前往另一栋大楼使用约克大学校园内另一台功能仪器的用户的堵塞。