UPLC System for Muscle Health Research

高效液相色谱法（UPLC）是一种使研究人员能够以极高的灵敏度分离、鉴定和定量生物样品成分的方法。它依靠压力使生物样品溶液通过一个柱。这种溶液可以与柱中存在的物质相互作用，并有效地将分子彼此分离，以帮助识别它们。在约克大学，我们有大量的研究人员在生物系，运动机能学和健康科学学院，肌肉健康研究中心（MHRC）需要使用UPLC技术。在这个申请中，我们请求资金来购买约克校区的第二个这样的设备系统。我们希望用它来分析氨基酸、儿茶酚胺、代谢物和多肽等生物样品，如血清、肌肉和心脏，主要是为了支持MHRC成员的研究，也为我们在其他部门的同事。我和我的5名共同申请人将大量使用这些设备，作为MHRC的主任，我也代表上述3个研究单位中从事细胞生物化学研究的众多（大约30名）其他教职员工提交这份申请，他们可能会使用这些设备。该设备将提高我们对复杂生物样品进行基础生化分析的能力，它的获得显著有助于消除用户目前前往约克大学校园另一栋建筑使用另一种功能仪器的僵局。

Ultra High-performance Liquid Chromatography (UPLC) is a method which allows the researcher to separate, identify and quantify the components of a biological sample with great sensitivity. It relies on the use of pressure to pass the biological sample solution through a column. This solution can interact with materials present in this column and effectively separate molecules from each other, to help identify them. At York University we have a large number of researchers in the Department of Biology, the School of Kinesiology and Health Science, and the Muscle Health Research Centre (MHRC) that require the use of UPLC technology. In this application we are requesting funds to acquire only the second such equipment system on the York campus. We wish to use this to analyze amino acids, catecholamines, metabolites and peptides in biological samples such a serum, muscle, and heart primarily to support the research of MHRC members, but also our colleagues in other departments. My 5 co-applicants and I will make heavy use of this equipment, and as the Director of the MHRC, I am submitting this application also on behalf of the numerous (>30) other faculty members in the 3 research units above who do cellular biochemistry research and could potentially make use of this equipment. This equipment will increase our capacity for the fundamental biochemical analyses of complex biological samples, and its acquisition markedly help to dissipate the logjam of users who currently travel to another building to use the one other functional instrument on the York University campus.