Flow Cytometer for Muscle Health Research

用于肌肉健康研究的流式细胞仪

• 批准号: RTI-2020-00599
• 负责人: Hood, David
• 金额: $ 10.91万
• 依托单位: York University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=693685
• 关键词: Flow; Cytometer; Muscle; Health; Research

******Flow Cytometry is a method which allows the researcher to examine the biochemical properties of individual cells, or organelles derived from cells, using fluorescent labeling techniques. It relies on the use of lasers which produce different wavelengths which can be used to excite and detect molecules within those cells or organelles. In this application, I am requesting funds to acquire only the third such equipment system on the entire York campus, and to replace my obsolete system which served us well in the past, but which now costs more to maintain than to use, and is now not operational. My trainees and colleagues will continue to use flow cytometry to analyze the biochemical properties of muscle cells in their immature (Myoblasts) and mature state (myotubes), as well as the characteristics of skin fibroblasts from healthy controls as well as mitochondrial myopathy patients. We also use flow cytometry to analyze the function of isolated mitochondria and mitochondrial phospholipids (eg. Cardiolipin) from healthy and exercised animal models, to support to support our NSERC Discovery grant objectives. Acquisition of this new equipment is absolutely essential for us to progress in achieving our NSERC DG research goals. A delay in its acquisition will have a significant impact on our progress, since we are waiting for a replacement. In addition, this equipment will have a significant impact on the training of HQP. My laboratory is in high demand for MSc, PhD and post-doctoral opportunities, and I currently have 12 trainees on a yearly basis. The flow cytometry system will allow me to train these HQP in marketable, sophisticated and up-to-date technology, which will serve them well in academic and other research and analysis settings. In addition, as the Director of the Muscle Health Research Centre (MHRC), I am also submitting this application to help support the >30 other faculty members in the Department of Biology, the School of Kinesiology and Health Science, and the MHRC, with the goal of increasing our currently very limited capacity for cellular analyses using this important methodology at York University. **

******流式细胞术是一种允许研究人员使用荧光标记技术检查单个细胞或源自细胞的细胞器的生化特性的方法。 它依赖于使用产生不同波长的激光，这些波长可用于激发和检测这些细胞或细​​胞器内的分子。在此申请中，我请求资金在整个约克校区购买第三个此类设备系统，并替换我的过时系统，该系统过去为我们提供了良好的服务，但现在维护成本高于使用成本，并且现在无法运行。我的学员和同事将继续使用流式细胞术来分析未成熟状态（成肌细胞）和成熟状态（肌管）的肌肉细胞的生化特性，以及来自健康对照和线粒体肌病患者的皮肤成纤维细胞的特征。 我们还使用流式细胞术分析健康和运动动物模型中分离的线粒体和线粒体磷脂（例如心磷脂）的功能，以支持我们的 NSERC 发现拨款目标。购买这种新设备对于我们实现 NSERC DG 研究目标绝对至关重要。延迟收购将对我们的进度产生重大影响，因为我们正在等待替代品。此外，该装备将对总部的训练产生重大影响。 我的实验室对硕士、博士和博士后机会的需求量很大，目前每年有 12 名学员。 流式细胞术系统将使我能够使用适销对路的、复杂的和最新的技术来培训这些 HQP，这将使他们在学术和其他研究和分析环境中得到很好的服务。 此外，作为肌肉健康研究中心 (MHRC) 的主任，我还提交此申请，以帮助支持生物系、运动学与健康科学学院和 MHRC 的其他 30 多名教职人员，目标是提高约克大学目前非常有限的使用这一重要方法进行细胞分析的能力。 **