Evaluation of adaptive significance of alternative splicing by phylogenetic conservation

通过系统发育保守评价选择性剪接的适应性意义

• 批准号: 238403-2013
• 负责人: Skandalis, Adonis
• 金额: $ 2.16万
• 依托单位: Brock University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=628857
• 关键词: Evaluation; adaptive; significance; alternative; splicing

Genetic information is stored in the cell in the form of DNA, which the cell cannot access directly. Instead, DNA information undergoes extensive, multistep processing before it can be acted upon to direct cellular functions. One of these processes, alternative mRNA splicing, not only transmits genetic information but also enhances it by generating multiple alternative genetic messages from the same gene. The cell expands considerable amount of resources to generate these alternative messages. While some are essential for the proper functioning and development of the cell, the significance of others is subject of great debate since their function in the cell is unclear. Are they errors tolerated by the cell or do they represent an as yet unknown type of genetic information or a novel regulatory process? In this study we will attempt to evaluate the significance of alternative messages by comparing their production in a variety of including mammals, birds, fish, and amphibians. Our guiding principle is that if alternative messages are functionally important then we will be able to trace their evolutionary development and uncover evidence of their role in the various animals we will investigate.

遗传信息以DNA的形式存储在细胞中，细胞无法直接访问。相反，DNA信息要经过广泛的、多步骤的处理，然后才能被用来指导细胞功能。其中一个过程--选择性信使核糖核酸剪接--不仅传递遗传信息，而且通过从同一基因产生多个选择性遗传信息来增强遗传信息。小区扩展相当数量的资源以生成这些可选消息。虽然有些对细胞的正常功能和发育是必不可少的，但由于它们在细胞中的功能尚不清楚，其他一些的意义仍存在很大争议。它们是细胞所能容忍的错误，还是代表了一种未知类型的遗传信息或一种新的调控过程？在这项研究中，我们将试图通过比较它们在包括哺乳动物、鸟类、鱼类和两栖动物在内的各种动物中的产生来评估替代信息的意义。我们的指导原则是，如果替代信息在功能上很重要，那么我们将能够追踪它们的进化发展，并发现它们在我们将研究的各种动物中扮演的角色的证据。