The role of Semaphorin 4C-PlexinB2 interaction in B-lymphocyte differentiation

Semaphorin 4C-PlexinB2 相互作用在 B 淋巴细胞分化中的作用

• 批准号: RGPIN-2017-06735
• 负责人: Mazer, Bruce
• 金额: $ 1.89万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=629258
• 关键词: role; Semaphorin; 4C; PlexinB2; interaction

B-lymphocytes are the cells that make antibodies, provide assistance to T-cells to help magnify immune responses against microbes and other foreign invaders, and produce chemicals called cytokines which transfer information from one cell to another. To improve the ability of B-lymphocytes to communicate, when B-cells are activated, important molecules on B-cells are brought to a pole on one side of the cell, in a structure known as the immune synapse. The molecules that control the formation of these synapses are not well studied. It is important to understand the regulation of the immune synapse to understand how cells communicate and potentially improve problems such as autoimmunity and antibody deficiencies. My laboratory discovered that a molecule known as Semaphorin 4C, thought to be only important in the nervous system, is produced when B-cells are activated to mature or to respond to particular types of foreign proteins. The full function of this molecule in B-cells is not known. However, our data suggests that Semaphorin 4C acts a part of the anchor allowing B-cells to properly form immune synapses. In mice that do not have this molecule, B-cells which are activated do not form normal immune synapses and their B-cells do not make normal amounts of antibodies or cytokines.

b淋巴细胞是制造抗体的细胞，为t细胞提供帮助，帮助放大针对微生物和其他外来入侵者的免疫反应，并产生一种叫做细胞因子的化学物质，这种化学物质将信息从一个细胞传递到另一个细胞。为了提高b淋巴细胞的沟通能力，当b细胞被激活时，b细胞上的重要分子被带到细胞一侧的极点上，这种结构被称为免疫突触。控制这些突触形成的分子还没有得到很好的研究。了解免疫突触的调节对于理解细胞如何沟通和潜在地改善自身免疫和抗体缺陷等问题是很重要的。我的实验室发现，当b细胞被激活成熟或对特定类型的外来蛋白质做出反应时，会产生一种被称为信号素4C的分子，这种分子被认为只对神经系统重要。这种分子在b细胞中的全部功能尚不清楚。然而，我们的数据表明，Semaphorin 4C作为锚点的一部分，允许b细胞正确地形成免疫突触。在没有这种分子的小鼠体内，被激活的b细胞不能形成正常的免疫突触，它们的b细胞不能产生正常数量的抗体或细胞因子。