High-resolution optical approaches to study dendritic signaling

研究树突信号传导的高分辨率光学方法

• 批准号: RGPIN-2017-06171
• 负责人: DeKoninck, Paul
• 金额: $ 2.91万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=629256
• 关键词: resolution; optical; approaches; study; dendritic

Understanding the mechanisms by which nerve cells transmit information and adapt their communication has progressed through the identification of the molecular components of synapses as well as the electrochemical nature of synaptic signals. However, for the majority of these components and signals, we know little about how they operate, propagate and interact during neurotransmission. To fully understand how synapses work, we must be able to resolve their molecular components and elementary signals within living neural circuits and to monitor them at length and time scales relevant to neurotransmission. Toward this aim, we have developed a Neurophotonics Research Program focussing on the development of advanced optical methods -in some cases beyond the resolution of light- to resolve the spatial and temporal dynamics of proteins and signals during synaptic transmission and to determine how these dynamics affect synaptic function. This Program is at the interface between Optics and Neurobiology. It bridges recent advances in optical methods, fluorescent probes, and cellular and molecular neuroscience. It trains a new generation of HQP in Biophotonics, supported by our Training Program in Biophotonics.

通过识别突触的分子成分以及突触信号的电化学性质，了解神经细胞传递信息和适应其通信的机制已经取得了进展。然而，对于大多数这些成分和信号，我们对它们在神经传递过程中如何运作、传播和相互作用知之甚少。为了充分理解突触的工作原理，我们必须能够解析它们的分子组成和活神经回路中的基本信号，并在与神经传递相关的长度和时间尺度上监测它们。为了实现这一目标，我们已经开发了一个神经光子学研究计划，专注于开发先进的光学方法-在某些情况下超越光的分辨率-以解决突触传递过程中蛋白质和信号的空间和时间动态，并确定这些动态如何影响突触功能。该计划是光学和神经生物学之间的接口。它连接了光学方法、荧光探针、细胞和分子神经科学的最新进展。它在生物光子学方面培训新一代HQP，由我们的生物光子学培训计划提供支持。