Mechanism and impact of de novo centriole assembly in mammalian cells

哺乳动物细胞中心粒从头组装的机制和影响

• 批准号: RGPIN-2015-05152
• 负责人: FitzHarris, Greg
• 金额: $ 2.48万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=629288
• 关键词: Mechanism; impact; de; novo; centriole

Centrioles dictate cell organisation and are instrumental in cell division. Centrioles replicate in S-phase, such that one pair is available to organize each pole of the mitotic spindle during cell division. Whilst replication of the existing centriole pair is the canonical mode of centriole generation, new centrioles can form ‘de-novo' if the existing centrioles are removed using lasers or microsurgery. Thus, cells have the capability to form new centrioles de novo, but very little is known about this pathway, and appropriate models for examining its mechanism are scarce.

中心粒决定细胞组织，并在细胞分裂中起作用。中心粒在S期复制，使得一对中心粒可用于在细胞分裂期间组织有丝分裂纺锤体的每个极。虽然现有中心粒对的复制是中心粒产生的典型模式，但如果使用激光或显微手术去除现有中心粒，则新的中心粒可以“从头”形成。因此，细胞具有从头形成新的中心粒的能力，但对这一途径知之甚少，并且用于检查其机制的适当模型很少。