Keratin intermediate filaments: a dynamic framework for major signaling molecules involved in epithelial cell survival and cell death

角蛋白中间丝：参与上皮细胞存活和细胞死亡的主要信号分子的动态框架

• 批准号: RGPIN-2016-05735
• 负责人: Cadrin, Monique
• 金额: $ 2.26万
• 依托单位: Université du Québec à Trois-Rivières
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=629455
• 关键词: Keratin; intermediate; filaments; dynamic; framework

Intermediate filaments (IFs) are with microtubules and actin microfilaments the major cytoskeletal components of most mammalian cells. Keratins are the IF proteins that are expressed in epithelial cells. A gene-targeting approach has been necessary to unravel the roles of keratin 8 and 18 in hepatocytes, the epithelial cells of the liver. For instance, hepatocytes from mice carrying K8 or K18 mutations that disrupt IF network structure or affect their phosphorylation pattern, and K8- or K18-null mice are highly susceptible to apoptosis induced by hepatotoxic agents. Thus K8/18 are proposed to play an important role in the protection of epithelial cells against stress-induced by toxicants. The mechanisms by which K8/18 accomplish their protective role is far from being fully understood.

中间丝（IFs）与微管和肌动蛋白微丝是大多数哺乳动物细胞的主要细胞骨架组成部分。角蛋白是在上皮细胞中表达的IF蛋白。一种基因靶向的方法已被必要的解开角蛋白8和18在肝细胞，肝的上皮细胞的作用。例如，来自携带破坏IF网络结构或影响其磷酸化模式的K8或K18突变的小鼠的肝细胞，以及K8或K18缺失小鼠对肝毒性剂诱导的细胞凋亡高度敏感。因此，K8/18被认为在保护上皮细胞免受毒物诱导的应激中起重要作用。K8/18实现其保护作用的机制远未完全了解。