Biophysical mechanisms driving spatial organization in bacterial cells

驱动细菌细胞空间组织的生物物理机制

• 批准号: RGPIN-2017-04435
• 负责人: Weber, Stephanie
• 金额: $ 3.28万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=629693
• 关键词: Biophysical; mechanisms; driving; spatial; organization

Living cells are divided into functional compartments called organelles. In eukaryotes, membranes create a diffusion barrier between organelles and the cytoplasm, such that each compartment maintains a distinct biochemical composition that is tailored for its particular function. The biophysical mechanisms underlying the structure and function of membrane-bound organelles are well understood. However, cells also contain organelles that are not enclosed by membranes. For example, stress granules, P bodies and the nucleolus belong to a class of membraneless organelles called "cellular bodies". These bodies consist of local concentrations of proteins and nucleic acids that rapidly exchange with the surrounding cytoplasm or nucleoplasm. Recent evidence suggests that cellular bodies assemble by liquid-liquid phase separation, during which soluble molecules condense from the cytoplasm (or nucleoplasm) to form concentrated liquid-like organelles. My postdoctoral work on the nucleolus, a large cellular body responsible for ribosome biogenesis, was instrumental in defining phase separation as a new mechanism for intracellular organization.

活细胞被分成称为细胞器的功能区室。在真核生物中，膜在细胞器和细胞质之间形成扩散屏障，使得每个隔室维持针对其特定功能定制的独特生化组成。膜结合细胞器的结构和功能的生物物理学机制已被充分理解。然而，细胞也含有不被膜包围的细胞器。例如，应激颗粒、P体和核仁属于一类称为“细胞体”的无膜细胞器。这些小体由局部集中的蛋白质和核酸组成，它们与周围的细胞质或核质迅速交换。最近的证据表明，细胞体通过液-液相分离组装，在此期间，可溶性分子从细胞质（或核质）浓缩形成浓缩的液体样细胞器。我的博士后工作是关于核仁，一个负责核糖体生物合成的大细胞体，这有助于将相分离定义为细胞内组织的新机制。