RNA helicase regulation of RNA metabolism and gene expression

RNA 解旋酶对 R​​NA 代谢和基因表达的调节

• 批准号: RGPIN-2016-05448
• 负责人: Owttrim, George
• 金额: $ 2.4万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=629721
• 关键词: RNA; helicase; regulation; metabolism; gene

All organisms must be able to sense and respond to changes in their environment. Two of the most commonly experienced stresses are changes in light and temperature, factors that are crucial for the survival of photosynthetic organisms. In response to these stresses, cells must alter protein abundance in order to survive. Proteins are produced from RNA in a process that requires the RNA to be folded into the correct 3-D structure. RNA binding proteins, termed RNA helicases, facilitate RNA folding in all organisms. The purpose of our research is to determine how changes in the growth environment regulate RNA helicase accumulation and how helicases interact with RNA. To accomplish this, we are using the photosynthetic model system, cyanobacteria, a potentially important source of biofuels and medically active compounds. Cyanobacteria contain a single RNA helicase whose abundance is elevated at 20

所有的有机体都必须能够感知并对环境的变化做出反应。最常见的两种压力是光和温度的变化，这两个因素对光合作用有机体的生存至关重要。为了应对这些压力，细胞必须改变蛋白质的丰度才能生存。蛋白质是从RNA中产生的，这一过程需要将RNA折叠成正确的三维结构。RNA结合蛋白，称为RNA解旋酶，促进所有生物体中的RNA折叠。我们研究的目的是确定生长环境的变化如何调节RNA解旋酶的积累，以及解旋酶如何与RNA相互作用。为了实现这一目标，我们正在使用光合作用模型系统--蓝藻，这是生物燃料和具有医疗活性的化合物的潜在重要来源。蓝藻含有单一的RNA解旋酶，其丰度在20时升高。