Plasmids and virulence in animal disease isolates of type A Clostridium perfringens

A 型产气荚膜梭菌动物疾病分离株的质粒和毒力

• 批准号: 183796-2011
• 负责人: Prescott, John
• 金额: $ 2.91万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=629737
• 关键词: Plasmids; virulence; animal; disease; isolates

There are a number of very important but not-well-characterized intestinal diseases of different animal species associated with the bacterium Clostridium perfringens, which are responsible for serious and often fatal illness. These range from commonly fatal diseases such as haemorrhagic abomasitis of cattle and typhlocolitis of horses, to less severe diseases such as neonatal enteritis of swine. The lack of characterization of these diseases rests on the uncertainty about the role of C. perfringens, and therefore uncertainties about diagnosis and control. It also leads to non-targeted use of antibiotics and thus potentially enhances antibiotic resistance. A key to the diversity and evolution of C. perfringens as a cause of serious intestinal disease is the unique family of species-restricted genetic structures called plasmids, which can move between strains by mating. The insertion of this region into a native plasmid has led to divergent development of the mating region, and to the later acquisition of new toxin and antibiotic resistance genes. This provides selective advantage to the bacterium and also results in its ability to adapt to cause disease in different animal species. The program of research will characterize the novel genetic regions and toxin genes associated these plasmids in three different important intestinal infections of animals (calves, pigs, horses) to understand how these are involved in disease, and how these diseases can be better diagnosed and controlled. This program has the potential to make a revolutionary change in understanding of C. perfringens as a cause of disease in animals, and may have important application for C. perfringens-associated intestinal diseases in people.

不同动物种属存在许多非常重要但特征不明确的肠道疾病，这些疾病与产气荚膜梭菌有关，可导致严重且往往致命的疾病。这些疾病包括常见的致命疾病，如牛的出血性皱胃炎和马的伤寒，也包括不太严重的疾病，如猪的新生儿肠炎。这些疾病的特征缺乏在于对C的作用的不确定性。产气荚膜杆菌，因此诊断和控制的不确定性。它还导致抗生素的非靶向使用，从而可能增强抗生素耐药性。对C.作为严重肠道疾病的原因的产气荚膜杆菌是称为质粒的物种限制性遗传结构的独特家族，其可以通过交配在菌株之间移动。将该区域插入天然质粒导致交配区域的分化发展，并导致后来获得新的毒素和抗生素抗性基因。这为细菌提供了选择性优势，也导致其适应不同动物物种的能力。该研究计划将描述与这些质粒相关的新的遗传区域和毒素基因在三种不同的重要动物肠道感染（小牛，猪，马）中的特征，以了解这些疾病如何参与疾病，以及如何更好地诊断和控制这些疾病。这个程序有可能使对C语言的理解发生革命性的变化。产气荚膜梭菌作为动物疾病的病因，并可能具有重要的应用。产气荚膜杆菌相关的肠道疾病。