Characterization of oral mucosal neural crest and mesoderm-derived connective tissue cells

口腔粘膜神经嵴和中胚层来源的结缔组织细胞的表征

• 批准号: RGPIN-2017-05765
• 负责人: Hakkinen, Lari
• 金额: $ 2.04万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=629915
• 关键词: Characterization; oral; mucosal; neural; crest

Cell interactions that lead to tooth formation are well understood but the processes involved in the development of oral mucosal gingiva—an anatomically, histologically and functionally distinct part of oral mucosa that is in close contact with teeth—have received little attention. Several studies have suggested that gingival connective tissue is composed of phenotypically distinct fibroblast subpopulations, but their identity has remained ambiguous. Interestingly, recent findings from our and other laboratories have indicated that gingival connective tissue cell subsets may derive from neural crest (NC) and mesoderm during development. Thus, the phenotypic heterogeneity of gingival fibroblast subpopulations may be determined by their developmental origin. We believe that gingiva provides an easily accessible source to study the fundamental properties of NC and mesoderm-derived cell subpopulations. Our general long-term goal is to identify and characterize these fibroblast subpopulations in gingiva and also use this information to understand the phenotype of fibroblast subsets in other tissues.

导致牙齿形成的细胞相互作用已被充分了解，但口腔粘膜牙龈（在解剖学、组织学和功能上与牙齿紧密接触的口腔粘膜的独特部分）发育所涉及的过程却很少受到关注。多项研究表明牙龈结缔组织由表型不同的成纤维细胞亚群组成，但它们的身份仍然不明确。有趣的是，我们和其他实验室的最新研究结果表明，牙龈结缔组织细胞亚群可能源自发育过程中的神经嵴（NC）和中胚层。因此，牙龈成纤维细胞亚群的表型异质性可能由其发育起源决定。我们相信牙龈为研究 NC 和中胚层来源的细胞亚群的基本特性提供了一个易于获取的来源。我们总体的长期目标是识别和表征牙龈中的这些成纤维细胞亚群，并利用这些信息来了解其他组织中成纤维细胞亚群的表型。