Characterization of "serosal bridges": routes for antigen and pathogen transport from the female reproductive tract to the intestines.

“浆膜桥”的表征：抗原和病原体从女性生殖道运输到肠道的途径。

• 批准号: 9375062
• 负责人: Vjollca H Konjufca
• 金额: $ 22.13万
• 依托单位: SOUTHERN ILLINOIS UNIVERSITY CARBONDALE
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-06-01 至 2019-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9375062
• 关键词: Animals; Antigen Targeting; Antigens; Azithromycin; Blood; Blood Circulation; Cells; Chlamydia; Chlamydia Infections; Chlamydia muridarum; Chlamydia trachomatis; Colon; Conceptions; Dendritic Cells; Development; Dextrans; Duodenum; Ectopic Pregnancy; Electron Microscopy; Endophthalmitis; Epithelial Cells; Equilibrium; Excision; FOXP3 gene; Female; Fetus; Gastrointestinal tract structure; HIV; Health; Hour; Human; Human Papillomavirus; IL2RA gene; ITGAX gene; Immune Tolerance; Immune response; Immunity; Immunization; Immunoglobulin A; Immunoglobulin G; Incidence; Infant; Infection; Infertility; Injectable; Intestinal Mucosa; Intestines; Lymphoid Follicle; M cell; Malignant neoplasm of cervix uteri; Mediating; Mesentery; Microscopy; Mucous Membrane; Mus; Neonatal; Ovalbumin; Pathway interactions; Pelvic Inflammatory Disease; Pneumonia; Pregnant Women; Regulatory T-Lymphocyte; Reporting; Reproductive Tract Infections; Research Personnel; Resistance; Risk; Role; Route; Sampling; Secretory Immunoglobulin A; Seminal fluid; Serous Membrane; Serum; Severity of illness; Sexually Transmitted Diseases; Site; Stomach; Structure; Structure of aggregated lymphoid follicle of small intestine; System; Tissues; Tunica Adventitia; Vaccination; Vagina; Vaginal Drug Administration; Woman; Work; base; cell motility; chronic pelvic pain; embryo/fetus antigen; fetal; interest; lymph nodes; migration; mucosal vaccine; nanoparticle; oral tolerance; pathogen; reproductive tract; three dimensional structure; transmission process; two-photon; vaccination strategy; vaccine development; young woman

PROJECT SUMMARY/ABSTRACT In this application the researchers propose to 1) characterize “serosal bridges” (SBs), a previously unknown internal route that facilitates antigen and cell transport between the female reproductive tract (FRT) and the gastro-intestinal (GI) tract; and 2) assess the essentiality of SBs and cell migration for transport of Chlamydia spp. from the FRT to the GI tract. The team showed that vaginal immunization with ovalbumin-conjugated 20 nm nanoparticles (NPs) induces serum IgG and primes the intestinal mucosa for secretion of IgA. Surprisingly, it was found that vaginally-instilled soluble antigens and NPs are “delivered” to the lower stomach and duodenum. Moreover, vaginally-instilled Chlamydia spp. elementary bodies (300-450 nm) reach SBs and intestinal serosa within 1-3 hours. In humans and animals Chlamydia spp. spreads from the FRT and persists in the GI tract, where it is resistant to azithromycin treatment. In mice, Chlamydia disseminates internally however, how it transits from the FRT to the GI tract remains unknown. Completion of the proposed work will be critical for understanding dissemination of Chlamydia from the FRT to the GI tract, as well as for developing vaccination strategies to target this pathogen.

项目总结/文摘