Characterization of "serosal bridges": routes for antigen and pathogen transport from the female reproductive tract to the intestines.

“浆膜桥”的表征：抗原和病原体从女性生殖道运输到肠道的途径。

• 批准号: 9375062
• 负责人: Vjollca H Konjufca
• 金额: $ 22.13万
• 依托单位: SOUTHERN ILLINOIS UNIVERSITY CARBONDALE
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-06-01 至 2019-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9375062
• 关键词: Animals; Antigen Targeting; Antigens; Azithromycin; Blood; Blood Circulation; Cells; Chlamydia; Chlamydia Infections; Chlamydia muridarum; Chlamydia trachomatis; Colon; Conceptions; Dendritic Cells; Development; Dextrans; Duodenum; Ectopic Pregnancy; Electron Microscopy; Endophthalmitis; Epithelial Cells; Equilibrium; Excision; FOXP3 gene; Female; Fetus; Gastrointestinal tract structure; HIV; Health; Hour; Human; Human Papillomavirus; IL2RA gene; ITGAX gene; Immune Tolerance; Immune response; Immunity; Immunization; Immunoglobulin A; Immunoglobulin G; Incidence; Infant; Infection; Infertility; Injectable; Intestinal Mucosa; Intestines; Lymphoid Follicle; M cell; Malignant neoplasm of cervix uteri; Mediating; Mesentery; Microscopy; Mucous Membrane; Mus; Neonatal; Ovalbumin; Pathway interactions; Pelvic Inflammatory Disease; Pneumonia; Pregnant Women; Regulatory T-Lymphocyte; Reporting; Reproductive Tract Infections; Research Personnel; Resistance; Risk; Role; Route; Sampling; Secretory Immunoglobulin A; Seminal fluid; Serous Membrane; Serum; Severity of illness; Sexually Transmitted Diseases; Site; Stomach; Structure; Structure of aggregated lymphoid follicle of small intestine; System; Tissues; Tunica Adventitia; Vaccination; Vagina; Vaginal Drug Administration; Woman; Work; base; cell motility; chronic pelvic pain; embryo/fetus antigen; fetal; interest; lymph nodes; migration; mucosal vaccine; nanoparticle; oral tolerance; pathogen; reproductive tract; three dimensional structure; transmission process; two-photon; vaccination strategy; vaccine development; young woman

PROJECT SUMMARY/ABSTRACT In this application the researchers propose to 1) characterize “serosal bridges” (SBs), a previously unknown internal route that facilitates antigen and cell transport between the female reproductive tract (FRT) and the gastro-intestinal (GI) tract; and 2) assess the essentiality of SBs and cell migration for transport of Chlamydia spp. from the FRT to the GI tract. The team showed that vaginal immunization with ovalbumin-conjugated 20 nm nanoparticles (NPs) induces serum IgG and primes the intestinal mucosa for secretion of IgA. Surprisingly, it was found that vaginally-instilled soluble antigens and NPs are “delivered” to the lower stomach and duodenum. Moreover, vaginally-instilled Chlamydia spp. elementary bodies (300-450 nm) reach SBs and intestinal serosa within 1-3 hours. In humans and animals Chlamydia spp. spreads from the FRT and persists in the GI tract, where it is resistant to azithromycin treatment. In mice, Chlamydia disseminates internally however, how it transits from the FRT to the GI tract remains unknown. Completion of the proposed work will be critical for understanding dissemination of Chlamydia from the FRT to the GI tract, as well as for developing vaccination strategies to target this pathogen.

项目摘要/摘要 在本应用程序中，研究人员提出的提议1）表征“浆膜桥”（SBS），这是一个以前未知的 促进女性生殖道（FRT）与细胞运输的内部途径 胃肠道（GI）道； 2）评估SBS和细胞迁移对衣原体的运输的本质 spp。从FRT到GI区。小组表明，卵蛋白结合的阴道免疫20 NM纳米颗粒（NPS）诱导血清IgG和肠道粘膜来分泌IgA。出奇， 已经发现，阴道灌输的固体抗原和NP被“递送”到下stalch， 十二指肠。此外，阴道灌输的衣原体属属。基本机构（300-450 nm）到达SBS和 肠血清1-3小时内。在人类和动物的衣原体中。从FRT蔓延并持续 在老鼠中，衣原体内部传播 但是，它如何从FRT转移到胃肠道仍然未知。拟议工作的完成将 对于了解将衣原体从FRT传播到胃肠道和发展至关重要 靶向这种病原体的疫苗接种策略。