Post-transcriptional control of gene expression and its role in plant responses to developmental and environmental cues

基因表达的转录后控制及其在植物对发育和环境线索的反应中的作用

• 批准号: RGPIN-2016-06505
• 负责人: Muench, Douglas
• 金额: $ 2.55万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=630363
• 关键词: Post; transcriptional; control; gene; expression

Plant gene expression is regulated by environmental and developmental signals that plants receive throughout their lifetime. The regulation of gene expression often occurs after genes are transcribed into RNA, in a process called post-transcriptional control. This process can regulate various aspects of RNA metabolism in the cell, including the stability of RNAs, their specific localization, and their translation into protein. RNA-binding proteins are critical mediators of post-transcriptional control of gene expression. They can directly regulate their target RNAs, or they can recruit other proteins that assist in RNA regulation. The long-term goal of my research program is to better understand the role of RNA-binding proteins in post-transcriptional control in plant cells, and how this form of gene regulation modulates plant development and plant responses to environmental signals. The short-term objectives of my research program, as outlined in this proposal, are aimed at understanding the molecular, biochemical and cellular functions of three RNA-binding proteins in the model plant, Arabidopsis thaliana (Arabidopsis). Two of these proteins belong to the PUF family of RNA-binding proteins. The other is a metabolic enzyme that possesses RNA binding activity. Because each of these proteins appear to have distinct roles in regulating the metabolism of RNAs, understanding how they work will provide valuable insight into the mechanisms of RNA-binding protein function in plant cells.

植物基因表达受植物一生中接受的环境和发育信号的调控。基因表达的调控通常发生在基因转录成RNA之后，这一过程称为转录后控制。这个过程可以调节细胞中RNA代谢的各个方面，包括RNA的稳定性，它们的特异性定位以及它们翻译成蛋白质。RNA结合蛋白是基因表达转录后调控的关键介质。它们可以直接调节其靶RNA，也可以招募其他蛋白质来辅助RNA调节。我的研究计划的长期目标是更好地了解RNA结合蛋白在植物细胞转录后控制中的作用，以及这种形式的基因调控如何调节植物发育和植物对环境信号的反应。我的研究计划的短期目标，如本提案所述，旨在了解模式植物拟南芥（Arabidopsis thaliana）中三种RNA结合蛋白的分子，生物化学和细胞功能。其中两种蛋白属于RNA结合蛋白的PUF家族。另一种是具有RNA结合活性的代谢酶。由于这些蛋白质中的每一种似乎在调节RNA代谢方面都具有不同的作用，因此了解它们的工作方式将为了解植物细胞中RNA结合蛋白功能的机制提供有价值的见解。