New microscale sample processing approaches for application to proteomics and bioanalysis

适用于蛋白质组学和生物分析的新型微量样品处理方法

• 批准号: RGPIN-2016-06636
• 负责人: Waldron, Karen
• 金额: $ 1.82万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=632581
• 关键词: New; microscale; sample; processing; approaches

The health and the well-being of Canadians rely not only on accurate diagnosis and effective treatment but also a pollution-free environment in which to live. Chemical analysis plays a key role in such goals by helping uncover the causes of disease, or by following a patient’s drug therapy response, or by testing water, soil and air for toxins to both plants and animals. By far the most common methods for said chemical analyses are grouped under the term “analytical separations”, encompassing the powerful techniques of chromatography, electrophoresis and mass spectrometry. The complexity of samples submitted for chemical analysis, sometimes containing over a thousand different compounds, is such that rigorous sample preparation techniques are required prior to the analytical separation. In fact, the selectivity and detection sensitivity of the above-mentioned analytical separation methods improve greatly with preparation techniques tailored to the analysis. Therefore, our research program is dedicated to developing and optimizing sample preparation methods with specific application to microliter scale bioanalysis and towards enhancing the power of state-of-the-art analytical separations.

加拿大人的健康和福祉不仅依赖于准确的诊断和有效的治疗，而且还依赖于无污染的生活环境。化学分析在这些目标中发挥着关键作用，有助于发现疾病的原因，或通过跟踪患者的药物治疗反应，或通过测试水，土壤和空气中的植物和动物毒素。到目前为止，用于所述化学分析的最常见的方法被归类在术语“分析分离”下，包括色谱法、电泳法和质谱法的强大技术。提交用于化学分析的样品的复杂性，有时包含超过一千种不同的化合物，使得在分析分离之前需要严格的样品制备技术。事实上，上述分析分离方法的选择性和检测灵敏度随着针对分析定制的制备技术而大大提高。因此，我们的研究计划致力于开发和优化样品制备方法，具体应用于微升规模的生物分析，并提高最先进的分析分离的能力。