MICROSCALE SAMPLE PREPARATION FOR MASS SPECTROMETRY

质谱分析的微量样品制备

• 批准号: 7955900
• 负责人: Catherine E. Costello
• 金额: $ 0.59万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955900
• 关键词: Biology; Cells; Chemicals; Computer Retrieval of Information on Scientific Projects Database; Digestion; Educational process of instructing; Educational workshop; Employment; Funding; Glycoside Hydrolases; Grant; Individual; Institution; Instruction; Laboratories; Mass Spectrum Analysis; Medicine; Methods; Peptide Hydrolases; Positioning Attribute; Preparation; Procedures; Proteins; Recovery; Research; Research Personnel; Resources; Sampling; Source; Techniques; Training; United States National Institutes of Health; Visit; base; experience; member; skills

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ms. Chan has a high level of expertise in the microscale manipulations of samples, including chemical transformations such as permethylation, peracetylation, HF cleavage, etc., as well as enzymatic degradations including both protease and glycosidase digestions and methods for sample cleanup. She trains new staff members to use these techniques. Ms. Wang is optimizing procedures for separation of proteins with the BioRad Mini-Prep Cell and is teaching others to perform the necessary manipulations. As new staff members join the laboratory, or present staff move on to new projects that require these skills, established group members provide group instruction and individual guidance so that the less experienced staff members can expedite their projects and gain expertise that will prove useful when they complete their postdoctoral or visiting positions and move on to more permanent employment. Training takes place on an individual basis or in a workshop format. Plans to conduct regular workshops continue. Training emphasizes sample recovery and a basic introduction into how samples should be analyzed.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 女士Chan在样品的微尺度操作方面具有高水平的专业知识，包括化学转化，如全甲基化，全乙酰化，HF裂解等，以及包括蛋白酶和糖苷酶二聚体的酶促降解和样品净化方法。她培训新员工使用这些技术。 王女士正在优化BioRad Mini-Prep Cell分离蛋白质的程序，并指导其他人进行必要的操作。随着新的工作人员加入实验室，或目前的工作人员转移到需要这些技能的新项目，建立小组成员提供小组指导和个人指导，使经验不足的工作人员可以加快他们的项目，并获得专业知识，这将证明是有用的，当他们完成博士后或访问职位，并继续更长期的就业。培训以个人或讲习班的形式进行。定期举办讲习班的计划仍在继续。培训强调样品回收和如何分析样品的基本介绍。