Antibody Glycosylation by Mass Spectrometry

通过质谱法进行抗体糖基化

• 批准号: RGPIN-2017-05502
• 负责人: Perreault, Helene
• 金额: $ 2.04万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=632592
• 关键词: Antibody; Glycosylation; Mass; Spectrometry

Antibodies (Abs) are glycoproteins of prime importance in the immune system, with immunoglobulin G (IgG) as their most common representative. There are many sources of IgGs and several motives to characterize their glycosylation patterns and amino acid (AA) sequences, which is the central theme of this proposal. The PI’s laboratory has specialized in glycosylation analysis for over 20 years, and has trained many HQP. This proposal involves a minimum of 10 HQP over 5 years and each of them will benefit of interdisciplinary training in bioanalytical chemistry and mass spectrometry (MS). Sections of the proposal are summarized below.

抗体（Abs）是免疫系统中最重要的糖蛋白，其中免疫球蛋白G （IgG）是最常见的代表。igg有许多来源，并且有几种动机来表征它们的糖基化模式和氨基酸（AA）序列，这是本提案的中心主题。PI实验室在糖基化分析方面有20多年的专业经验，培养了许多HQP。该计划涉及至少10名HQP，为期5年，每个HQP都将受益于生物分析化学和质谱（MS）的跨学科培训。建议的各节摘要如下。