Expanding the use of high-speed sCMOS cameras into selective 3-dimensional imaging

高速摄像机提供了前所未有的实时观察生物活细胞过程的能力，以及制造过程中的机器视觉。在这两种情况下，都可以得到三维物体的二维图像序列。访问二维（X-Y）平面之外的实时信息，即X、Y和Z维度，是一个挑战。典型的共聚焦扫描显微镜解决了三维可视化的问题。然而，这种显微镜价格昂贵（约50万美元），而且在涉及高速离面运动的过程中能力有限。获取三维实时信息一直是我们的商业合作伙伴OptikonCorporation Ltd热衷于解决的一个挑战。为了解决这个问题，提议的合作工作旨在建立一个程序，使用已知的组件-一个科学的CMOS相机（sCMOS），以及高速聚焦光学-获得3D信息，而不需要真正的共聚焦扫描。该提案的终点将是一份技术应用说明，使未来来自商业合作伙伴Optikon Corporation Ltd.的sCMOS相机购买者能够获得关于如何将高速聚焦光学器件与sCMOS相机集成的明确指导方针，以便能够实时可视化X，Y和Z中的多个区域。

High-speed cameras have provided unprecedented ability to look at biological live-cell processes in real time aswell as machine vision in manufacturing processes. In both cases, one obtains two-dimensional image series ofthree-dimensional objects. Accessing real-time information outside a two-dimensional (X-Y) plane, i.e., in X,Y, and Z dimensions, is a challenge. Typical confocal scanning microscopes address the problem ofvisualization in 3 dimensions. However, such microscopes are expensive (of order $500,000) and, nevertheless,have limited capabilities for processes that involve high-speed out-of-plane motions.Accessing real-time information in three dimensions has been a challenge that our commercial partner, OptikonCorporation Ltd., is keen to resolve. To solve this problem, the proposed collaborative work aims to establish aprocedure that uses known components - a scientific CMOS camera (sCMOS), along with high speed focusingoptics - to get 3D information without requiring true confocal scanning. The end point of this proposal wouldbe a Technical Application Note that enables future purchasers of sCMOS cameras from the commercialpartner, Optikon Corporation Ltd., to have access to clear guidelines on how to integrate high-speed focusingoptics with a sCMOS camera in order to be able to visualize multiple regions in X,Y and Z in real time.