Characterization and selection of advanced potato varieties with multiple resistance genes against late blight

晚疫病多重抗性马铃薯先进品种的鉴定与筛选

• 批准号: 508706-2017
• 负责人: Joly, David
• 金额: $ 1.82万
• 依托单位: Université de Moncton
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=639322
• 关键词: Characterization; selection; advanced; potato; varieties

Semences Élites du Québec Inc. is a producer of elite seed potatoes located in a protected area. As such, potatolate blight, caused by Phytophthora infestans, represent a constant threat that they must keep under control inorder to keep their certification. Potato late blight is usually controlled through an intense fungicide sprayregime, which raises serious health and environmental issues, and is not option for seed potato growers inprotected areas and/or organic producers. The use of resistant varieties represents one of the mostenvironmentally-friendly alternative, still none of the potato varieties commercially available in Canada haveadequate levels of resistance against late blight. This project aims at characterizing advanced potato varietiesfrom Semences Élites du Québec Inc. to identify those harboring multiple genes of resistance against lateblight. This will be achieved through the use of molecular markers linked with known resistance genes,together with a state-of-the-art effectoromics approach, which consist of directly testing the ability of a givenvariety to recognize a particular avirulence gene from the pathogen. This last approach could result in theidentification of novel or unknown sources of resistance, and will be helpful to identify resistance genes forwhich molecular markers do not exist. Identifying resistant varieties for release will help the company maintainphytosanitary conditions in protected areas, as well as allowing them to select resistant varieties forpropagation and final stages of field testing.

魁北克精英研讨会是一家位于保护区内的优质马铃薯种子生产商。因此，马铃薯枯萎病，引起的致病疫霉，代表了一个不断的威胁，他们必须保持控制，以保持他们的认证。马铃薯晚疫病通常通过强烈的杀真菌剂喷洒制度来控制，这会引起严重的健康和环境问题，并且不是保护区和/或有机生产者的种薯种植者的选择。使用抗性品种代表了一种对环境最友好的替代方法，但在加拿大商业上可获得的马铃薯品种仍然没有足够的抗晚疫病水平。该项目旨在表征来自魁北克省高级马铃薯品种。来鉴定那些携带多个抗晚疫病基因的植物。这将通过使用与已知抗性基因相关的分子标记以及最先进的效应组学方法来实现，该方法包括直接测试给定品种识别病原体中特定无毒基因的能力。最后一种方法可能导致鉴定新的或未知的抗性来源，并将有助于鉴定分子标记不存在的抗性基因。确定用于释放的抗性品种将有助于该公司改善保护区的植物检疫条件，并允许他们选择抗性品种进行繁殖和田间试验的最后阶段。